ArticleJournal of bacteriology2009
The autolysin LytA contributes to efficient bacteriophage progeny release in Streptococcus pneumoniae.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- A Novel Lytic Podovirus AP-20-A InfectingInternational journal of molecular sciences · 2026Article
- Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance.Poultry science · 2026Article
- Structure, Function, and Regulation of LytA: TheMicroorganisms · 2025Review
- Article
- Cell wall modifications that alter the exolytic activity of lactococcal phage endolysins have little impact on phage growth.Frontiers in microbiology · 2023Article
- Bacteriocin-phage interaction (BaPI): Phage predation of Lactococcus in the presence of bacteriocins.MicrobiologyOpen · 2022Article
- A Novel and Ubiquitous Marine Methylophage Provides Insights into Viral-Host Coevolution and Possible Host-Range Expansion in Streamlined Marine Heterotrophic Bacteria.Applied and environmental microbiology · 2022Article
- Article
- Lysogeny inMicroorganisms · 2020Review
- How Phages Overcome the Challenges of Drug Resistant Bacteria in Clinical Infections.Infection and drug resistance · 2020Review
- Interactions between Bacteriophages and Eukaryotic Cells.Scientifica · 2020Review
- Enzymes and Mechanisms Employed by Tailed Bacteriophages to Breach the Bacterial Cell Barriers.Viruses · 2018Review
- Tolerance of a phage element by Streptococcus pneumoniae leads to a fitness defect during colonization.Journal of bacteriology · 2014Article
- Article
- Transduction of the Streptococcus pyogenes bacteriophage Φm46.1, carrying resistance genes mef(A) and tet(O), to other Streptococcus species.Frontiers in microbiology · 2014Article
- Structural basis of the novel S. pneumoniae virulence factor, GHIP, a glycosyl hydrolase 25 participating in host-cell invasion.PloS one · 2013Article
- A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.The Journal of biological chemistry · 2012Article
- Prophage spontaneous activation promotes DNA release enhancing biofilm formation in Streptococcus pneumoniae.PloS one · 2010Article
- Evidence of localized prophage-host recombination in the lytA gene, encoding the major pneumococcal autolysin.Journal of bacteriology · 2010Article
- Lytic bacteriophages as alternative to overcoming antibiotic-resistant biofilms formed by clinically significant bacteria.Therapeutic advances in infectious diseaseReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful bacterial autolysin LytA in virion release is currently unknown. In this study, deletions of the bacterial and phage lysins were done in lysogenic S. pneumoniae strains, allowing the evaluation of the contribution of each lytic enzyme to phage release through the monitoring of bacterial-culture lysis and phage plaque assays. In addition, we assessed membrane integrity during phage-mediated lysis using flow cytometry to evaluate the regulatory role of holins over the lytic activities. Our data show that LytA is activated at the end of the lytic cycle and that its triggering results from holin-induced membrane permeabilization. In the absence of phage lysin, LytA is able to mediate bacterial lysis and phage release, although exclusive dependence on the autolysin results in reduced virion egress and altered kinetics that may impair phage fitness. Under normal conditions, activation of bacterial LytA, together with the phage lysin, leads to greater phage progeny release. Our findings demonstrate that S. pneumoniae phages use the ubiquitous host autolysin to accomplish an optimal phage exiting strategy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.