ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2009
Measurement of one and two bond N-C couplings in large proteins by TROSY-based J-modulation experiments.
Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Solution Domain Dynamics of Monomeric SARS-CoV‑2 Main Protease Revealed by Optimized NMR Residual Dipolar Coupling Measurements.ACS physical chemistry Au · 2026Article
- Coherence locking in a parallel nuclear magnetic resonance probe defends against gradient field spillover.Magnetic resonance (Gottingen, Germany) · 2025Article
- A J-modulated protonless NMR experiment characterizes the conformational ensemble of the intrinsically disordered protein WIP.Journal of biomolecular NMR · 2016Article
- Glycosylation Alters Dimerization Properties of a Cell-surface Signaling Protein, Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1).The Journal of biological chemistry · 2016Article
- Hybrid Approaches to Structural Characterization of Conformational Ensembles of Complex Macromolecular Systems Combining NMR Residual Dipolar Couplings and Solution X-ray Scattering.Chemical reviews · 2016Review
- An encodable lanthanide binding tag with reduced size and flexibility for measuring residual dipolar couplings and pseudocontact shifts in large proteins.Journal of biomolecular NMR · 2016Article
- Structure of decorin binding protein B from Borrelia burgdorferi and its interactions with glycosaminoglycans.Biochimica et biophysica acta · 2015Article
- The Structural Role of Antibody N-Glycosylation in Receptor Interactions.Structure (London, England : 1993) · 2015Article
- Structural characterization of a flexible two-domain protein in solution using small angle X-ray scattering and NMR data.Structure (London, England : 1993) · 2014Article
- Solution NMR structures of immunoglobulin-like domains 7 and 12 from obscurin-like protein 1 contribute to the structural coverage of the Human Cancer Protein Interaction Network.Journal of structural and functional genomics · 2014Article
- Solution NMR structure of CD1104B from pathogenic Clostridium difficile reveals a distinct α-helical architecture and provides first structural representative of protein domain family PF14203.Journal of structural and functional genomics · 2013Article
- NMR methods for structural studies of large monomeric and multimeric proteins.Current opinion in structural biology · 2013Review
- The magic of bicelles lights up membrane protein structure.Chemical reviews · 2012Review
- Solution structure of decorin-binding protein A from Borrelia burgdorferi.Biochemistry · 2012Article
- Lanthanide binding and IgG affinity construct: potential applications in solution NMR, MRI, and luminescence microscopy.Protein science : a publication of the Protein Society · 2012Article
- Expanding the utility of NMR restraints with paramagnetic compounds: background and practical aspects.Progress in nuclear magnetic resonance spectroscopy · 2011Review
- Improved accuracy in measuring one-bond and two-bond (15)N, (13)C (α) coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy.Journal of biomolecular NMR · 2011Article
- Structures of domains I and IV from YbbR are representative of a widely distributed protein family.Protein science : a publication of the Protein Society · 2011Article
- Calmodulin regulates Ca2+-sensing receptor-mediated Ca2+ signaling and its cell surface expression.The Journal of biological chemistry · 2010Article
- A device for the measurement of residual chemical shift anisotropy and residual dipolar coupling in soluble and membrane-associated proteins.Journal of biomolecular NMR · 2010Article
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Abstract
Residual dipolar couplings (RDCs) between NC' and NC(alpha) atoms in polypeptide backbones of proteins contain information on the orientation of bond vectors that is complementary to that contained in NH RDCs. The (1)J(NC)(alpha) and (2)J(NC)(alpha) scalar couplings between these atoms also display a Karplus relation with the backbone torsion angles and report on secondary structure. However, these N-C couplings tend to be small and they are frequently unresolvable in frequency domain spectra having the broad lines characteristic of large proteins. Here a TROSY-based J-modulated approach for the measurement of small (15)N-(13)C couplings in large proteins is described. The cross-correlation interference effects inherent in TROSY methods improve resolution and signal to noise ratios for large proteins, and the use of J-modulation to encode couplings eliminates the need to remove frequency distortions from overlapping peaks during data analysis. The utility of the method is demonstrated by measurement of (1)J(NC'), (1)J(NC)(alpha) , and (2)J(NC)(alpha) scalar couplings and (1)D(NC') and D(NC)(alpha) residual dipolar couplings for the myristoylated yeast ARF1.GTPgammas protein bound to small lipid bicelles, a system with an effective molecule weight of approximately 70kDa.
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