ArticleDevelopmental biology2009
Isl1 is a direct transcriptional target of Forkhead transcription factors in second-heart-field-derived mesoderm.
Article in Developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- ISL1 common variant rs1017 is not associated with susceptibility to congenital heart disease in a Chinese population.Genetic testing and molecular biomarkers · 2012Trial
- Genetic knockout suggests Isl1 enhancer redundancy in mouse hindlimb development.Developmental biology · 2025Article
- Single-cell landscape identified SERPINB9 as a key player contributing to stemness and metastasis in non-seminomas.Cell death & disease · 2024Article
- Sall genes regulate hindlimb initiation in mouse embryos.Genetics · 2024Article
- TAPVR: Molecular Pathways and Animal Models.Advances in experimental medicine and biology · 2024Review
- Human pluripotent stem cell fate trajectories toward lung and hepatocyte progenitors.iScience · 2023Article
- The Tbx20-TLE interaction is essential for the maintenance of the second heart field.Development (Cambridge, England) · 2023Article
- In Vitro Embryogenesis and Gastrulation Using Stem Cells in Mice and Humans.International journal of molecular sciences · 2023Review
- The second heart field: the first 20 years.Mammalian genome : official journal of the International Mammalian Genome Society · 2023Review
- Spotlight on Isl1: A Key Player in Cardiovascular Development and Diseases.Frontiers in cell and developmental biology · 2021Review
- ATAC-Seq Reveals anCirculation research · 2020Article
- A novel cardiomyogenic role for Isl1Science advances · 2020Article
- The deployment of cell lineages that form the mammalian heart.Nature reviews. Cardiology · 2018Review
- Gata6 restricts Isl1 to the posterior of nascent hindlimb buds through Isl1 cis-regulatory modules.Developmental biology · 2018Article
- Single-cell analysis delineates a trajectory toward the human early otic lineage.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Review
- Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.Stem cells (Dayton, Ohio) · 2015Article
- Heart fields and cardiac morphogenesis.Cold Spring Harbor perspectives in medicine · 2014Review
- The functional diversity of essential genes required for mammalian cardiac development.Genesis (New York, N.Y. : 2000) · 2014Review
- Wnt-promoted Isl1 expression through a novel TCF/LEF1 binding site and H3K9 acetylation in early stages of cardiomyocyte differentiation of P19CL6 cells.Molecular and cellular biochemistry · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The cells of the second heart field (SHF) contribute to the outflow tract and right ventricle, as well as to parts of the left ventricle and atria. Isl1, a member of the LIM-homeodomain transcription factor family, is expressed early in this cardiac progenitor population and functions near the top of a transcriptional pathway essential for heart development. Isl1 is required for the survival and migration of SHF-derived cells into the early developing heart at the inflow and outflow poles. Despite this important role for Isl1 in early heart formation, the transcriptional regulation of Isl1 has remained largely undefined. Therefore, to identify transcription factors that regulate Isl1 expression in vivo, we screened the conserved noncoding sequences from the mouse Isl1 locus for enhancer activity in transgenic mouse embryos. Here, we report the identification of an enhancer from the mouse Isl1 gene that is sufficient to direct expression to the SHF and its derivatives. The Isl1 SHF enhancer contains three consensus Forkhead transcription factor binding sites that are efficiently and specifically bound by Forkhead transcription factors. Importantly, the activity of the enhancer is dependent on these three Forkhead binding sites in transgenic mouse embryos. Thus, these studies demonstrate that Isl1 is a direct transcriptional target of Forkhead transcription factors in the SHF and establish a transcriptional pathway upstream of Isl1 in the SHF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.