ArticleBiophysical journal2009
Cell adhesion molecule distribution relative to neutrophil surface topography assessed by TIRFM.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Improving vasculoprotective effects of MSCs in coronary microvessels - benefits of 3D culture, sub-populations and heparin.Frontiers in immunology · 2023Article
- Single-molecule imaging and microfluidic platform reveal molecular mechanisms of leukemic cell rolling.Communications biology · 2021Article
- Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
- Endothelial Glycocalyx Layer Properties and Its Ability to Limit Leukocyte Adhesion.Biophysical journal · 2020Article
- Microfluidics-based super-resolution microscopy enables nanoscopic characterization of blood stem cell rolling.Science advances · 2018Article
- Mapping cell surface adhesion by rotation tracking and adhesion footprinting.Scientific reports · 2017Article
- Controlled One-on-One Encounters between Immune Cells and Microbes Reveal Mechanisms of Phagocytosis.Biophysical journal · 2015Review
- Cell surface topography is a regulator of molecular interactions during chemokine-induced neutrophil spreading.Biophysical journal · 2014Article
- Highly permeable silicon membranes for shear free chemotaxis and rapid cell labeling.Lab on a chip · 2014Article
- Dynamics of adhesion molecule domains on neutrophil membranes: surfing the dynamic cell topography.European biophysics journal : EBJ · 2013Article
- Quantifying the mechanical properties of the endothelial glycocalyx with atomic force microscopy.Journal of visualized experiments : JoVE · 2013Article
- Tangential tether extraction and spontaneous tether retraction of human neutrophils.Biophysical journal · 2012Article
- 'Slings' enable neutrophil rolling at high shear.Nature · 2012Article
- An optical method to quantify the density of ligands for cell adhesion receptors in three-dimensional matrices.Journal of the Royal Society, Interface · 2010Article
- Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling.Nature methods · 2010Article
- Nanotopographical modification: a regulator of cellular function through focal adhesions.Nanomedicine : nanotechnology, biology, and medicine · 2010Review
- The transmembrane domains of L-selectin and CD44 regulate receptor cell surface positioning and leukocyte adhesion under flow.The Journal of biological chemistry · 2010Article
- Identifying the rules of engagement enabling leukocyte rolling, activation, and adhesion.PLoS computational biology · 2010Article
- Molecular accessibility in relation to cell surface topography and compression against a flat substrate.Biophysical journal · 2009Article
- Use of TIRF to Monitor T-Lymphocyte Membrane Dynamics with Submicrometer and Subsecond Resolution.Cellular and molecular bioengineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The positioning of adhesion molecules relative to the microtopography of the cell surface has a significant influence on the molecule's availability to form adhesive contacts. Measurements of the ratio of fluorescence intensity per unit area in epi-fluorescence images versus total internal reflection fluorescence images provides a means to assess the relative accessibility for bond formation of different fluorescently labeled molecules in cells pressed against a flat substrate. Measurements of the four principal adhesion molecules on human neutrophils reveal that L-selectin has the highest ratio of total internal reflection fluorescence/epi intensity, and that P-selectin glycoprotein ligand-1 (PSGL-1) and the integrins alphaLbeta2 (LFA-1) and alphaMbeta2 (Mac-1) have ratios similar to each other but lower than for L-selectin. All of the ratios increased with increasing impingement, indicating an alteration of surface topography with increasing surface compression. These results are consistent with model predictions for molecules concentrated near the tips of microvilli in the case of L-selectin, and sequestered away from the microvillus tips in the case of LFA-1, Mac-1, and PSGL-1. The results confirm differences among adhesion molecules in their surface distribution and reveal how the availability of specific adhesion molecules is altered by mechanical compression of the surface in live cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.