ArticleBiophysical journal2009
Multiscale measurements distinguish cellular and interstitial hindrances to diffusion in vivo.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.
- Collagenase-mediated extracellular matrix targeting for enhanced drug penetration and therapeutic efficacy in nanoscale delivery systems for cancer therapy.Journal of nanobiotechnology · 2025Pooled it
- Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow.Journal of biomedical optics · 2023Article
- Therapeutic Strategies to Overcome Fibrotic Barriers to Nanomedicine in the Pancreatic Tumor Microenvironment.Cancers · 2023Review
- Chirality-enhanced transport and drug delivery of graphene nanocarriers to tumor-like cellular spheroid.Frontiers in chemistry · 2023Article
- PEGPH20, a PEGylated human hyaluronidase, induces radiosensitization by reoxygenation in pancreatic cancer xenografts. A molecular imaging study.Neoplasia (New York, N.Y.) · 2022Article
- Review
- The Use of Alternative Strategies for Enhanced Nanoparticle Delivery to Solid Tumors.Chemical reviews · 2021Review
- Identification of peptide coatings that enhance diffusive transport of nanoparticles through the tumor microenvironment.Nanoscale · 2019Article
- Second-harmonic generation directionality is associated with neoadjuvant chemotherapy response in breast cancer core needle biopsies.Journal of biomedical optics · 2019Article
- Electro-Acupuncture Promotes Accumulation of Paclitaxel by Altering Tumor Microvasculature and Microenvironment in Breast Cancer of Mice.Frontiers in oncology · 2019Article
- Fluorescence techniques in developmental biology.Journal of biosciences · 2018Review
- Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions.Biomedical optics express · 2018Article
- Review
- Conceptual and Experimental Tools to Understand Spatial Effects and Transport Phenomena in Nonlinear Biochemical Networks Illustrated with Patchy Switching.Annual review of biochemistry · 2017Review
- Modelling drug transport during intraperitoneal chemotherapy.Pleura and peritoneum · 2017Review
- Cancer and inflammation.Wiley interdisciplinary reviews. Systems biology and medicine · 2017Review
- Modulating the Tumor Microenvironment to Enhance Tumor Nanomedicine Delivery.Frontiers in pharmacology · 2017Review
- Delivery of cancer therapeutics to extracellular and intracellular targets: Determinants, barriers, challenges and opportunities.Advanced drug delivery reviews · 2016Review
- Stromal barriers and strategies for the delivery of nanomedicine to desmoplastic tumors.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Interstitial flows promote amoeboid over mesenchymal motility of breast cancer cells revealed by a three dimensional microfluidic model.Integrative biology : quantitative biosciences from nano to macro · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Molecular cancer therapy relies on interstitial diffusion for drug distribution in solid tumors. A mechanistic understanding of how tumor components affect diffusion is necessary to advance cancer drug development. Yet, because of limitations in current techniques, it is unclear how individual tissue components hinder diffusion. We developed multiscale fluorescence recovery after photobleaching (MS-FRAP) to address this deficiency. Diffusion measurements facilitated by MS-FRAP distinguish the diffusive hindrance of the interstitial versus cellular constituents in living tissue. Using multiscale diffusion measurements in vivo, we resolved the contributions of these two major tissue components toward impeding diffusive transport in solid tumors and subcutaneous tissue in mice. We further used MS-FRAP in interstitial matrix-mimetic gels and in vivo to show the influence of physical interactions between collagen and hyaluronan on diffusive hindrance through the interstitium. Through these studies, we show that interstitial hyaluronan paradoxically improves diffusion and that reducing cellularity enhances diffusive macromolecular transport in solid tumors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.