ArticleBiophysical journal2009
Biophysical characterization of styryl dye-membrane interactions.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
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- Lipopolysaccharide clustering in colistin persistent and resistant bacteria.npj antimicrobials and resistance · 2025Article
- A practical protocol for correlative confocal fluorescence and transmission electron microscopy characterization of extracellular vesicles.Microbiology spectrum · 2025Article
- Decoding Bacterial Motility: From Swimming States to Patterns and Chemotactic Strategies.Biomolecules · 2025Review
- β2-Adrenergic Regulation of the Neuromuscular Transmission and Its Lipid-Dependent Switch.Molecular neurobiology · 2024Article
- Fluorescent Chiral Quantum Dots to Unveil Origin-Dependent Exosome Uptake and Cargo Release.ACS applied bio materials · 2024Article
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- Facilitating flip-flop: Structural tuning of molecule-membrane interactions in living bacteria.Biophysical journal · 2023Article
- Frequency-Dependent Engagement of Synaptic Vesicle Pools in the Mice Motor Nerve Terminals.Cellular and molecular neurobiology · 2023Article
- Live-Cell Imaging of the Assembly and Ejection Processes of the Bacterial Flagella by Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Laser-induced microinjury of the corneal basal epithelium and imaging of resident macrophage responses in a live, whole-eye preparation.Frontiers in immunology · 2023Article
- Mutations inFrontiers in microbiology · 2022Article
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- Use of in vitro bone models to screen for altered bone metabolism, osteopathies, and fracture healing: challenges of complex models.Archives of toxicology · 2020Review
- Construction and Loss of Bacterial Flagellar Filaments.Biomolecules · 2020Review
- Second Harmonic Generation Spectroscopy of Membrane Probe Dynamics in Gram-Positive Bacteria.Biophysical journal · 2019Article
- Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake.The Journal of cell biology · 2019Article
- Generating Giant Membrane Vesicles from Live Cells with Preserved Cellular Properties.Research (Washington, D.C.) · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Styryl dyes (also referred to as FM dyes) become highly fluorescent upon binding to membranes and are often used to study synaptic vesicle recycling in neurons. To date, however, no direct comparisons of the fluorescent properties, or time-resolved (millisecond) measurements of dye-membrane binding and unbinding reactions, for all members of this family of probes have been reported. Here, we compare the fluorescence intensities of each member of the FM dye family when bound to membranes. This analysis included SGC5, a new lipophilic fluorescent dye with a unique structure. Fluorescence intensities depended on the length of the lipophilic tail of each dye, with a rank order as follows: SGC5 > FM1-84 > FM1-43 > SynaptoGreen C3 > FM2-10/FM4-64/FM5-95. Stopped-flow measurements revealed that dye hydrophobicity determined the affinity and departitioning rates for dye-membrane interactions. All of the dyes dissociated from membranes on the millisecond timescale, which is orders of magnitude faster than the overall destaining rate (timescale of seconds) of these dyes from presynaptic boutons. Departitioning kinetics were faster at higher temperatures, but were unaffected by pH or cholesterol. The data reported here aid interpretation of dye-release kinetics from single synaptic vesicles, and indicate that these probes dissociate from membranes on more rapid timescales than previously appreciated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.