ArticleDiabetes technology & therapeutics2009
Transfection of rat pancreatic islet tissue by polymeric gene vectors.
Article in Diabetes technology & therapeutics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- eVLP-Mediated Cas9 Delivery for Preventing IBMIR in Islet Transplantation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Epigallocatechin gallate attenuated high glucose-induced pancreatic beta cell dysfunction by modulating DRP1-mediated mitochondrial apoptosis pathways.Scientific reports · 2024Article
- RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src.Scientific reports · 2016Article
- Protein-Binding Function of RNA-Dependent Protein Kinase Promotes Proliferation through TRAF2/RIP1/NF-κB/c-Myc Pathway in Pancreatic β cells.Molecular medicine (Cambridge, Mass.) · 2015Article
- Nucleotide sequencing, cloning, and expression of Capra hircus Heme Oxygenase-1 in caprine islets to promote insulin secretion in vitro.Molecular biotechnology · 2015Article
- MST1 is a key regulator of beta cell apoptosis and dysfunction in diabetes.Nature medicine · 2014Article
- The effect of environmental pH on polymeric transfection efficiency.Biomaterials · 2012Article
- Trafficking microenvironmental pHs of polycationic gene vectors in drug-sensitive and multidrug-resistant MCF7 breast cancer cells.Biomaterials · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIn vitro genetic modification has been regarded as one option to improve the viability and functionality of pancreatic islets when used for transplantation in patients with diabetes, either as naked islets or in a type of bioartificial pancreas. In this approach, vector safety and poor transfection efficiency are major concerns.
methodsIn this study, the influence of in vitro transfection conditions on polyplexes constructed of polyethyleneimine (PEI) and plasmid DNA (pDNA) on the transfection efficiency was investigated by varying the transfection medium, the pDNA dose, and the amines of polycation/phosphates of pDNA (N/P) ratio.
resultsCa2+-containing Krebs-Ringer-HEPES medium was more effective than RPMI 1640 medium by increasing transfection efficiency (2.5-fold). An increase in pDNA dose slightly reduced the transfection efficiency but had minimal influence on islet loss. However, the N/P ratio had a large effect on islet viability and transfection efficiency. For example, the PEI/pDNA ratio at N/P = 10 caused greater islet loss (56% vs. 28%) and 30-fold less transfection efficiency than at N/P = 5. Even under a set of best conditions selected from this study, mostly a fraction of cells located in the peripheral regions of an islet were transfected, and the viability and insulin secretion from the treated islets were not altered. However, it was found that the extent of apoptosis was noticeably higher (approximately 16%) than in untreated islets (approximately 2%).
conclusionsThese results suggest that the gene delivery efficacy to isolated islets can be improved by manipulating the transfection conditions. Polymeric vectors will broaden the options for islet transfection, which is currently limited to viral vectors.
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