ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2009
High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barley.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 69 citations in OpenAlex.
- Current knowledge and breeding strategies for management of aphid-transmitted viruses of pepper (Frontiers in plant science · 2024Review
- Upgrading the genome of an elite japonica rice variety Kongyu 131 for lodging resistance improvement.Plant biotechnology journal · 2023Article
- Genotyping and Multivariate Regression Trees Reveal Ecological Diversification within the Microcystis aeruginosa Complex along a Wide Environmental Gradient.Applied and environmental microbiology · 2022Article
- Identification of genetic loci and candidate genes related to β-glucan content in barley grain by genome-wide association study in International Barley Core Selected Collection.Molecular breeding : new strategies in plant improvement · 2021Article
- Updating the elite rice variety Kongyu 131 by improving the Gn1a locus.Rice (New York, N.Y.) · 2017Article
- Sequence diversification in recessive alleles of two host factor genes suggests adaptive selection for bymovirus resistance in cultivated barley from East Asia.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Genetic analyses of BaMMV/BaYMV resistance in barley accession HOR4224 result in the identification of an allele of the translation initiation factor 4e (Hv-eIF4E) exclusively effective against Barley mild mosaic virus (BaMMV).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- A series of eIF4E alleles at the Bc-3 locus are associated with recessive resistance to Clover yellow vein virus in common bean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Viral and cellular factors involved in Phloem transport of plant viruses.Frontiers in plant science · 2013Article
- Developing tools for investigating the multiple roles of ethylene: identification and mapping genes for ethylene biosynthesis and reception in barley.Molecular genetics and genomics : MGG · 2012Article
- Eukaryotic translation initiation factor 4E-mediated recessive resistance to plant viruses and its utility in crop improvement.Molecular plant pathology · 2012Review
- High-resolution melting analysis as a powerful tool to discriminate and genotype Pseudomonas savastanoi pathovars and strains.PloS one · 2012Article
- High resolution melting analysis for the detection of EMS induced mutations in wheat SBEIIa genes.BMC plant biology · 2011Article
- EcoTILLING in Capsicum species: searching for new virus resistances.BMC genomics · 2010Article
- High-resolution melting-based TILLING of γ ray-induced mutations in rice.Journal of Zhejiang University. Science. BArticle
- Frequency and type of inheritable mutations induced by γ rays in rice as revealed by whole genome sequencing.Journal of Zhejiang University. Science. BArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
An original method has been established for the identification of novel alleles of eukaryotic translation initiation factor 4E (eIF4E) gene, which is required for resistance to agronomically important bymoviruses, in barley germplasm. This method involves scanning for sequence variations in cDNA-derived PCR amplicons using High-resolution melting (HRM) followed by direct Sanger sequencing of only those amplicons which were predicted to carry nucleotide changes. HRM is a simple, cost-effective, rapid and high-throughput assay, which so far has only been widely used in clinical pathology for molecular diagnostic of diseases and patient genotyping. Application of HRM allowed significant reduction in the amount of expensive Sanger sequencing required for allele mining in plants. The method described here involved an investigation of total cDNA rather than genomic DNA, thus permitting the analyses of shorter (up to 300-bp) and fewer overlapping amplicons to cover the coding sequence. This strategy further reduced the allele mining costs. The sensitivity and accuracy of HRM for predicting genotypes carrying a wide range of nucleotide polymorphisms in eIF4E approached 100%. Results of the current study are promising and suggest that this method could also potentially be applied to the discovery of superior alleles controlling other important traits in barley as well in other model and crop plant species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.