ArticlePLoS pathogens2009
The alphaGal epitope of the histo-blood group antigen family is a ligand for bovine norovirus Newbury2 expected to prevent cross-species transmission.
Article in PLoS pathogens, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 88 citations in OpenAlex.
- Clinical and Epidemiological Features of Calicivirus Infections in Cattle.Animals : an open access journal from MDPI · 2026Review
- Immunogenicity of a bivalent recombinant adenovirus expressing VP1 proteins of bovine norovirus and bovine nebovirus in mice and calves.BMC microbiology · 2025Article
- Unlocking the secrets of Feline calicivirus: advances in structural and nonstructural proteins and its role as a key model for other Caliciviruses.Virology journal · 2025Review
- Virus-Glycan Interactions Studied by Solute NMR.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- NMR Experiments Shed New Light on Glycan Recognition by Human and Murine Norovirus Capsid Proteins.Viruses · 2021Article
- Human Noroviruses Attach to Intestinal Tissues of a Broad Range of Animal Species.Journal of virology · 2021Article
- Histo-blood group antigens as divergent factors of groups A and C rotaviruses circulating in humans and different animal species.Emerging microbes & infections · 2020Article
- A unique combination of glycoside hydrolases inThe Journal of biological chemistry · 2020Article
- Fondness for sugars of enteric viruses confronts them with human glycans genetic diversity.Human genetics · 2020Review
- The Coxsackievirus and Adenovirus Receptor, a Required Host Factor for Recovirus Infection, Is a Putative Enteric Calicivirus Receptor.Journal of virology · 2019Article
- Dual Recognition of Sialic Acid and αGal Epitopes by the VP8* Domains of the Bovine Rotavirus G6P[5] WC3 and of Its Mono-reassortant G4P[5] RotaTeq Vaccine Strains.Journal of virology · 2019Article
- GII.13/21 Noroviruses Recognize Glycans with a Terminal β-Galactose via an Unconventional Glycan Binding Site.Journal of virology · 2019Article
- Animals as Reservoir for Human Norovirus.Viruses · 2019Review
- Recipients with blood group A associated with longer survival rates in cardiac valvular bioprostheses.EBioMedicine · 2019Article
- Virus recognition of glycan receptors.Current opinion in virology · 2019Review
- Absence of Neu5Gc and Presence of Anti-Neu5Gc Antibodies in Humans-An Evolutionary Perspective.Frontiers in immunology · 2019Review
- Structural basis of host ligand specificity change of GII porcine noroviruses from their closely related GII human noroviruses.Emerging microbes & infections · 2019Article
- Article
- Bovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens.Journal of virology · 2018Article
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Authors and funding
8 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among Caliciviridae, the norovirus genus encompasses enteric viruses that infect humans as well as several animal species, causing gastroenteritis. Porcine strains are classified together with human strains within genogroup II, whilst bovine norovirus strains represent genogroup III. Various GI and GII human strains bind to carbohydrates of the histo-blood group family which may be shared among mammalian species. Genetic relatedness of human and animal strains as well as the presence of potentially shared ligands raises the possibility of norovirus cross-species transmission. In the present study, we identified a carbohydrate ligand for the prototype bovine norovirus strain Bo/Newbury2/76/UK (NB2). Attachment of virus-like particles (VLPs) of the NB2 strain to bovine gut tissue sections showed a complete match with the staining by reagents recognizing the Galalpha1,3 motif. Alpha-galactosidase treatment confirmed involvement of a terminal alpha-linked galactose. Specific binding of VLPs to the alphaGal epitope (Galalpha3Galbeta4GlcNAcbeta-R) was observed. The binding of Galalpha3GalalphaOMe to rNB2 VLPs was characterized at atomic resolution employing saturation transfer difference (STD) NMR experiments. Transfection of human cells with an alpha1,3galactosyltransferase cDNA allowed binding of NB2 VLPs, whilst inversely, attachment to porcine vascular endothelial cells was lost when the cells originated from an alpha1,3galactosyltransferase KO animal. The alphaGal epitope is expressed in all mammalian species with the exception of the Hominidaea family due to the inactivation of the alpha1,3galactosyltransferase gene (GGTA1). Accordingly, the NB2 carbohydrate ligand is absent from human tissues. Although expressed on porcine vascular endothelial cells, we observed that unlike in cows, it is not present on gut epithelial cells, suggesting that neither man nor pig could be infected by the NB2 bovine strain.
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