ArticlePLoS pathogens2009
Evidence for a "wattle and daub" model of the cyst wall of entamoeba.
Article in PLoS pathogens, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 68 citations in OpenAlex.
- Trafficking and organization of cyst wall components into a robust biphasic structure in Entamoeba.PLoS pathogens · 2026Article
- Cellulose binding and the timing of expression influence protein targeting to the double-layered cyst wall ofmSphere · 2024Article
- Characterization of Entamoeba fatty acid elongases; validation as targets and provision of promising leads for new drugs against amebiasis.PLoS pathogens · 2024Article
- Genetic characterization of theParasitology · 2024Article
- Novel Insights into the Wattle and Daub Model ofPathogens (Basel, Switzerland) · 2023Article
- Glycogen Metabolism and Its Role in Growth and Encystation inLife (Basel, Switzerland) · 2023Article
- Article
- The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba.Molecular biology and evolution · 2021Article
- Encystation ofMicroorganisms · 2021Article
- Stage-SpecificmSphere · 2021Article
- The dynamics of ultrastructural changes duringParasitology · 2020Article
- The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls.PLoS neglected tropical diseases · 2019Article
- Demonstration and Characterization of Cyst-Like Structures in the Life Cycle ofFrontiers in cellular and infection microbiology · 2019Article
- An NADeLife · 2018Article
- High-Throughput Screening ofFrontiers in cellular and infection microbiology · 2018Article
- A Flow Cytometry Method for Dissecting the Cell Differentiation Process ofFrontiers in cellular and infection microbiology · 2018Article
- Flow cytometric characterization of encystation in Entamoeba invadens.Molecular and biochemical parasitology · 2017Article
- Membrane Trafficking Modulation during Entamoeba Encystation.Scientific reports · 2017Article
- Entamoeba Encystation: New Targets to Prevent the Transmission of Amebiasis.PLoS pathogens · 2016Review
- A Structurally Novel Chitinase from the Chitin-Degrading Hyperthermophilic Archaeon Thermococcus chitonophagus.Applied and environmental microbiology · 2016Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The cyst wall of Entamoeba invadens (Ei), a model for the human pathogen Entamoeba histolytica, is composed of fibrils of chitin and three chitin-binding lectins called Jacob, Jessie3, and chitinase. Here we show chitin, which was detected with wheat germ agglutinin, is made in secretory vesicles prior to its deposition on the surface of encysting Ei. Jacob lectins, which have tandemly arrayed chitin-binding domains (CBDs), and chitinase, which has an N-terminal CBD, were each made early during encystation. These results are consistent with their hypothesized roles in cross-linking chitin fibrils (Jacob lectins) and remodeling the cyst wall (chitinase). Jessie3 lectins likely form the mortar or daub of the cyst wall, because 1) Jessie lectins were made late during encystation; 2) the addition to Jessie lectins to the cyst wall correlated with a marked decrease in the permeability of cysts to nucleic acid stains (DAPI) and actin-binding heptapeptide (phalloidin); and 3) recombinant Jessie lectins, expressed as a maltose-binding proteins in the periplasm of Escherichia coli, caused transformed bacteria to agglutinate in suspension and form a hard pellet that did not dissociate after centrifugation. Jessie3 appeared as linear forms and rosettes by negative staining of secreted recombinant proteins. These findings provide evidence for a "wattle and daub" model of the Entamoeba cyst wall, where the wattle or sticks (chitin fibrils likely cross-linked by Jacob lectins) is constructed prior to the addition of the mortar or daub (Jessie3 lectins).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.