ArticleActa pharmacologica Sinica2009
High K+-induced contraction requires depolarization-induced Ca2+ release from internal stores in rat gut smooth muscle.
Article in Acta pharmacologica Sinica, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
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- Characterization of calcium sources and downstream signaling pathways following electrical field stimulation of rodent longitudinal esophageal muscle.Frontiers in physiology · 2026Article
- Contractions of ex-vivo mouse bladder driven by activation of channelrhodopsin-2 expressed in urothelial cells.Scientific reports · 2025Article
- Age-dependent effects of the βPflugers Archiv : European journal of physiology · 2024Article
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- Antispasmodic Effect of Asperidine B, a Pyrrolidine Derivative, through Inhibition of L-Type CaMolecules (Basel, Switzerland) · 2021Article
- Analysis of KThe Malaysian journal of medical sciences : MJMS · 2021Article
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- Dandelion extract relaxes mouse airway smooth muscle by blocking VDLCC and NSCC channels.Cell & bioscience · 2020Article
- Involvement of inhibitor kappa B kinase 2 (IKK2) in the regulation of vascular tone.Laboratory investigation; a journal of technical methods and pathology · 2018Article
- Antispasmodic Effect of Blackcurrant (Ribes nigrum L.) Juice and Its Potential Use as Functional Food in Gastrointestinal Disorders.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2018Article
- The Role of Visceral Hypersensitivity in Irritable Bowel Syndrome: Pharmacological Targets and Novel Treatments.Journal of neurogastroenterology and motility · 2016Review
- P2Y receptor-mediated transient relaxation of rat longitudinal ileum preparations involves phospholipase C activation, intracellular Ca(2+) release and SK channel activation.Acta pharmacologica Sinica · 2016Article
- Cortex phellodendri Extract Relaxes Airway Smooth Muscle.Evidence-based complementary and alternative medicine : eCAM · 2016Article
- Relaxant action of plumula nelumbinis extract on mouse airway smooth muscle.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Effects of ginsenoside Re on rat jejunal contractility.Journal of natural medicines · 2014Article
- Age-dependent contribution of Rho kinase in carbachol-induced contraction of human detrusor smooth muscle in vitro.Acta pharmacologica Sinica · 2014Article
- Main ion channels and receptors associated with visceral hypersensitivity in irritable bowel syndrome.Annals of gastroenterology · 2014Review
- Inositol trisphosphate receptors in smooth muscle cells.American journal of physiology. Heart and circulatory physiology · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimDepolarization-induced contraction of smooth muscle is thought to be mediated by Ca2+ influx through voltage-gated L-type Ca2+channels. We describe a novel contraction mechanism that is independent of Ca2+ entry.
methodsPharmacological experiments were carried out on isolated rat gut longitudinal smooth muscle preparations, measuring isometric contraction strength upon high K+-induced depolarization.
resultsTreatment with verapamil, which presumably leads to a conformational change in the channel, completely abolished K+-induced contraction, while residual contraction still occurred when Ca2+ entry was blocked with Cd2+. These results were further confirmed by measuring intracellular Ca2+ transients using Fura-2. Co-application of Cd2+ and the ryanodine receptor blocker DHBP further reduced contraction, albeit incompletely. Additional blockage of either phospholipase C (U 73122) or inositol 1,4,5-trisphophate (IP3)receptors (2-APB) abolished most contractions, while sole application of these blockers and Cd2+ (without parallel ryanodine receptor manipulation) also resulted in incomplete contraction block.
conclusionWe conclude that there are parallel mechanisms of depolarization-induced smooth muscle contraction via (a) Ca2+ entry and (b) Ca2+ entry-independent, depolarization-induced Ca2+-release through ryanodine receptors and IP3, with the latter being dependent on phospholipase C activation.
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