ArticleJournal of the American Society for Mass Spectrometry2009
Top-down proteomics reveals novel protein forms expressed in Methanosarcina acetivorans.
Article in Journal of the American Society for Mass Spectrometry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Proteoforms expand the world of microproteins and short open reading frame-encoded peptides.iScience · 2023Review
- A Practical Guide to Small Protein Discovery and Characterization Using Mass Spectrometry.Journal of bacteriology · 2022Article
- A comprehensive pipeline for translational top-down proteomics from a single blood draw.Nature protocols · 2019Article
- Abundant Lysine Methylation and N-Terminal Acetylation in Sulfolobus islandicus Revealed by Bottom-Up and Top-Down Proteomics.Molecular & cellular proteomics : MCP · 2016Article
- Progress in Top-Down Proteomics and the Analysis of Proteoforms.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Review
- Molecular characterization of the thioredoxin system from Methanosarcina acetivorans.The FEBS journal · 2014Article
- Top Down proteomics: facts and perspectives.Biochemical and biophysical research communications · 2014Review
- Top-down proteomics with mass spectrometry imaging: a pilot study towards discovery of biomarkers for neurodevelopmental disorders.PloS one · 2014Article
- Rapid characterization of microorganisms by mass spectrometry--what can be learned and how?Journal of the American Society for Mass Spectrometry · 2013Review
- Article
- Extreme challenges and advances in archaeal proteomics.Current opinion in microbiology · 2012Review
- Protein identification using top-down.Molecular & cellular proteomics : MCP · 2012Article
- Nano-LC FTICR tandem mass spectrometry for top-down proteomics: routine baseline unit mass resolution of whole cell lysate proteins up to 72 kDa.Analytical chemistry · 2012Article
- Evaluation of direct infusion-multiple reaction monitoring mass spectrometry for quantification of heat shock proteins.Analytical chemistry · 2012Article
- Analysis of intact protein isoforms by mass spectrometry.The Journal of biological chemistry · 2011Review
- Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium.Analytical biochemistry · 2011Article
- Sub-part-per-million precursor and product mass accuracy for high-throughput proteomics on an electron transfer dissociation-enabled orbitrap mass spectrometer.Molecular & cellular proteomics : MCP · 2010Article
- Multi-dimensional liquid chromatography in proteomics--a review.Analytica chimica acta · 2010Review
- Identification and characterization of sORF-encoded polypeptides.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Using both automated nanospray and online liquid chromatography mass spectrometry LC-MS strategies, 99 proteins have been newly identified by top-down tandem mass spectrometry (MS/MS) in Methanosarcina acetivorans, the methanogen with the largest known genome [5.7 mega base pairs (Mb)] for an Archaeon. Because top-down MS/MS was used, 15 proteins were detected with mispredicted start sites along with an additional five from small open reading frames (SORFs). Beyond characterization of these more common discrepancies in genome annotation, one SORF resulted from a rare start codon (AUA) as the initiation site for translation of this protein. Also, a methylation on a 30S ribosomal protein (MA1259) was localized to Pro59-Val69, contrasting sharply from its homologue in Escherichia coli (rp S12) known to harbor an unusual beta-thiomethylated aspartic acid residue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.