ArticleThe Journal of surgical research2009
Diurnal expression and function of peptide transporter 1 (PEPT1).
Article in The Journal of surgical research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Hypothyroidism impairs the circadian rhythmicity of clock genes and proteins involved in gut nutrient absorption in female mice.Frontiers in physiology · 2025Article
- Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transport.Structure (London, England : 1993) · 2022Article
- Nutrient timing and metabolic regulation.The Journal of physiology · 2022Review
- The pathways and mechanisms of muramyl dipeptide transcellular transport mediated by PepT1 in enterogenous infection.Annals of translational medicine · 2019Article
- Circadian rhythms: a regulator of gastrointestinal health and dysfunction.Expert review of gastroenterology & hepatology · 2019Review
- Di- and tripeptide transport in vertebrates: the contribution of teleost fish models.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Review
- Expression and regulation of the neutral amino acid transporter B0AT1 in rat small intestine.PloS one · 2017Article
- Diurnal rhythmicity in biological processes involved in bioavailability of functional food factors.Journal of clinical biochemistry and nutrition · 2013Article
- Comparative digestive physiology.Comprehensive Physiology · 2013Review
- Peptide absorption after massive proximal small bowel resection: mechanisms of ileal adaptation.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2011Article
- Differentiating passive from transporter-mediated uptake by PepT1: a comparison and evaluation of four methods.The Journal of surgical research · 2011Article
- Intestinal adaptation for oligopeptide absorption via PepT1 after massive (70%) mid-small bowel resection.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2011Article
- Pharmacologic considerations for oseltamivir disposition: focus on the neonate and young infant.Paediatric drugs · 2011Review
- Role of vagal innervation in diurnal rhythm of intestinal peptide transporter 1 (PEPT1).Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2009Article
- Expression and function of intestinal hexose transporters after small intestinal denervation.Surgery · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundProtein is absorbed primarily as di/tripeptides, which are transported into the enterocyte exclusively by H(+)/peptide cotransporter 1 (PEPT1). Diurnal changes in expression and function of several other mucosal transporters occur in rat. Diurnal variations in mRNA, protein, and transport function of PEPT1 occur in rat duodenum and jejunum, but not in ileum.
methodsMucosal levels of mRNA and protein were determined at 9 AM, 3 PM, 9 PM, and 3 AM (n=6 each) by real time RT-PCR and Western blotting, respectively, in rats maintained in a 12-h light/dark room [light 6 AM to 6 PM]; transporter-mediated uptake of dipeptide (Gly-Sar) was also measured by everted sleeve technique.
resultsmRNA transcripts of PEPT1 and Gly-Sar uptake varied diurnally in duodenum and jejunum (peak at 3 PM, P<0.05), but not in ileum; maximal uptake was in jejunum. V(max) (nmol/cm/min) was greater at 3 PM and 9 PM compared with 9 AM (3 PM versus 9 AM: 104 versus 62 in duodenum, and 185 versus 101 in jejunum; P<0.03); K(m) was unchanged across time points or locations. Protein levels varied minimally in jejunum and ileum with peaks at 9 PM and 3 AM.
conclusionGene expression and transport function of PEPT1 vary diurnally in duodenum and jejunum in temporal association with nocturnal feeding of rats.
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