ArticleAntiviral research2009
Effective inhibition of hepatitis E virus replication in A549 cells and piglets by RNA interference (RNAi) targeting RNA-dependent RNA polymerase.
Article in Antiviral research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Combining RNA Interference and RIG-I Activation to Inhibit Hepatitis E Virus Replication.Viruses · 2024Article
- Characteristics and Functions of HEV Proteins.Advances in experimental medicine and biology · 2023Article
- An RNA Interference/Adeno-Associated Virus Vector-Based Combinatorial Gene Therapy Approach Against Hepatitis E Virus.Hepatology communications · 2022Article
- Influenza A Virus Production in Mouse Lung Is Inhibited by Inhalation of Aerosol Polyethylenimine/Short Hairpin RNA Plasmid Complexes.Disease markers · 2022Article
- Hepatitis E Virus Replication.Viruses · 2019Review
- Successful infection of BALB/c mice by a swine hepatitis E virus clone constructed with reverse genetics.BMC infectious diseases · 2018Article
- A review on current status of antiviral siRNA.Reviews in medical virology · 2018Review
- Hepatitis E virus infection activates signal regulator protein α to down-regulate type I interferon.Immunologic research · 2016Article
- Molecular Biology and Infection of Hepatitis E Virus.Frontiers in microbiology · 2016Review
- Therapeutic targets for the treatment of hepatitis E virus infection.Expert opinion on therapeutic targets · 2015Review
- Anti-HBV efficacy of combined siRNAs targeting viral gene and heat shock cognate 70.Virology journal · 2012Article
- RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells.Virology journal · 2012Article
- Inhibition of Hazara nairovirus replication by small interfering RNAs and their combination with ribavirin.Virology journal · 2011Article
- RNA interference induces effective inhibition of mRNA accumulation and protein expression of SHEV ORF3 gene in vitro.Current microbiology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is a natural mechanism for suppressing or silencing expression of aberrant or foreign genes. It is a powerful antiviral strategy that has been widely employed to protect hosts from viral infection. Hepatitis E (HE) is an acute fulminant hepatitis in adults that has particularly high mortality in pregnant women. At this point in time, there is no vaccine or antiviral treatment that is effective against the infectious agent, HEV. The nonstructural polyprotein region possesses an RNA-dependent RNA polymerase (RdRp) that is responsible for the replication of the viral RNA genome. RdRp is therefore regarded as one of the most attractive candidates for RNA interference (RNAi). In the present study, the high efficiency and specificity of siRNA were evaluated by Real-Time quantitative PCR and Western blot assays. Protective effects against HEV infection were achieved in A549 cells and in piglets. In piglets treated with a shRNA-RdRp-1 expression plasmid prior to HEV inoculation, HEV antigens were significantly reduced in the liver, spleen, and kidneys, and the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) were clearly decreased. These results suggested that RNAi is a potentially effective antiviral strategy against HEV replication and infection.
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Registered trials
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