ArticleThe EMBO journal2009
Concerted action of two cation filters in the aquaporin water channel.
Article in The EMBO journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 98 citations in OpenAlex.
- Intrinsic Asymmetry in Weak Acid Transmembrane Transporters.Biomolecules · 2026Review
- Independent divergences of the aquaporins across different genera highlight the distinct adaptation mechanisms within Mytilida.BMC genomics · 2025Article
- The Expanding Role of Aquaporin-1, Aquaporin-3 and Aquaporin-5 as Transceptors: Involvement in Cancer Development and Potential Druggability.International journal of molecular sciences · 2025Review
- Protein Structures of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Characterization of human aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery.Bioscience reports · 2024Article
- Characterization of SIPs-type aquaporins and their roles in response to environmental cues in rice (Oryza sativa L.).BMC plant biology · 2024Article
- Transcendent Aspects of Proton Channels.Annual review of physiology · 2024Review
- Methylthiosulfonate-Based Cysteine Modifiers as Alternative Inhibitors of Mercurial-Sensitive Aquaporins.Cells · 2023Article
- Comparison of ion selectivities of nitrite channel NirC and water channel aquaporin.World journal of microbiology & biotechnology · 2023Article
- Aquaporin Gating: A New Twist to Unravel Permeation through Water Channels.International journal of molecular sciences · 2022Review
- Multiple pore lining residues modulate water permeability of GlpF.Protein science : a publication of the Protein Society · 2022Article
- Time-Dependent Biosensor Fluorescence as a Measure of Bacterial Arsenic Uptake Kinetics and Its Inhibition by Dissolved Organic Matter.Applied and environmental microbiology · 2022Article
- The Multifaceted Role of Aquaporin-9 in Health and Its Potential as a Clinical Biomarker.Biomolecules · 2022Review
- Mitogen-Activated Protein Kinase and Aquaglyceroporin Gene Expression in Treatment Failure Leishmania major.Acta parasitologica · 2022Article
- Aquaporin ion conductance properties defined by membrane environment, protein structure, and cell physiology.Biophysical reviews · 2022Review
- Structure and function of a silicic acid channel Lsi1.Frontiers in plant science · 2022Review
- The energetic barrier to single-file water flow through narrow channels.Biophysical reviews · 2021Review
- AQPX-cluster aquaporins and aquaglyceroporins are asymmetrically distributed in trypanosomes.Communications biology · 2021Article
- Aquaporins in Cereals-Important Players in Maintaining Cell Homeostasis under Abiotic Stress.Genes · 2021Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aquaporin (AQP) facilitated water transport is common to virtually all cell membranes and is marked by almost perfect specificity and high flux rates. Simultaneously, protons and cations are strictly excluded to maintain ionic transmembrane gradients. Yet, the AQP cation filters have not been identified experimentally. We report that three point mutations turned the water-specific AQP1 into a proton/alkali cation channel with reduced water permeability and the permeability sequence: H(+) >>K(+) >Rb(+) >Na(+) >Cs(+) >Li(+). Contrary to theoretical models, we found that electrostatic repulsion at the central asn-pro-ala (NPA) region does not suffice to exclude protons. Full proton exclusion is reached only in conjunction with the aromatic/arginine (ar/R) constriction at the pore mouth. In contrast, alkali cations are blocked by the NPA region but leak through the ar/R constriction. Expression of alkali-leaking AQPs depolarized membrane potentials and compromised cell survival. Our results hint at the alkali-tight but solute-unselective NPA region as a feature of primordial channels and the proton-tight and solute-selective ar/R constriction variants as later adaptations within the AQP superfamily.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.