ArticleProceedings of the National Academy of Sciences of the United States of America2009
Development of GFP-based biosensors possessing the binding properties of antibodies.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Chagas Disease Diagnosis withVaccines · 2024Article
- Review
- Loading characteristics of streptavidin on polypropylene capillary channeled polymer fibers and capture performance towards biotinylated proteins.Analytical and bioanalytical chemistry · 2023Article
- Protein engineering via sequence-performance mapping.Cell systems · 2023Review
- A Thermal-Stable Protein Nanoparticle That Stimulates Long Lasting Humoral Immune Response.Vaccines · 2023Article
- DogCatcher allows loop-friendly protein-protein ligation.Cell chemical biology · 2022Article
- Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever.Scientific reports · 2021Article
- Functional display of bioactive peptides on the vGFP scaffold.Scientific reports · 2021Article
- Engineering Cell-Permeable Proteins through Insertion of Cell-Penetrating Motifs into Surface Loops.ACS chemical biology · 2020Article
- High specificity of widely used phospho-tau antibodies validated using a quantitative whole-cell based assay.Journal of neurochemistry · 2020Article
- Evolution of C-Terminal Modification Tolerance in Full-Length and Split T7 RNA Polymerase Biosensors.Chembiochem : a European journal of chemical biology · 2019Article
- Stoichiometry and compositional plasticity of the yeast nuclear pore complex revealed by quantitative fluorescence microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Integrin-Targeting Fluorescent Proteins: Exploration of RGD Insertion Sites.Chembiochem : a European journal of chemical biology · 2017Article
- Affinity Maturation of a Cyclic Peptide Handle for Therapeutic Antibodies Using Deep Mutational Scanning.The Journal of biological chemistry · 2017Article
- A 45-Amino-Acid Scaffold Mined from the PDB for High-Affinity Ligand Engineering.Chemistry & biology · 2015Article
- Thermal green protein, an extremely stable, nonaggregating fluorescent protein created by structure-guided surface engineering.Proteins · 2015Article
- The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Applications of Yeast Surface Display for Protein Engineering.Methods in molecular biology (Clifton, N.J.) · 2015Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Proteins that can bind specifically to targets that also have an intrinsic property allowing for easy detection could facilitate a multitude of applications. While the widely used green fluorescent protein (GFP) allows for easy detection, attempts to insert multiple binding loops into GFP to impart affinity for a specific target have been met with limited success because of the structural sensitivity of the GFP chromophore. In this study, directed evolution using a surrogate loop approach and yeast surface display yielded a family of GFP scaffolds capable of accommodating 2 proximal, randomized binding loops. The library of potential GFP-based binders or ''GFAbs'' was subsequently mined for GFAbs capable of binding to protein targets. Identified GFAbs bound with nanomolar affinity and required binding contributions from both loops indicating the advantage of a dual loop GFAb platform. Finally, GFAbs were solubly produced and used as fluorescence detection reagents to demonstrate their utility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.