ArticleAnalytical chemistry2009
Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- Cost-effective fabrication of photopolymer molds with multi-level microstructures for PDMS microfluidic device manufacture.RSC advances · 2020Article
- A multivariate, quantitative assay that disentangles key kinetic parameters of primary human T cell function in vitro.PloS one · 2020Article
- An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells.Bio-protocol · 2018Article
- Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification.Technology · 2018Article
- Time-resolved, single-cell analysis of induced and programmed cell death via non-invasive propidium iodide and counterstain perfusion.Scientific reports · 2016Article
- Phenotypic drug profiling in droplet microfluidics for better targeting of drug-resistant tumors.Lab on a chip · 2015Article
- The microfluidic multitrap nanophysiometer for hematologic cancer cell characterization reveals temporal sensitivity of the calcein-AM efflux assay.Scientific reports · 2014Article
- Rapid prototyping of concave microwells for the formation of 3D multicellular cancer aggregates for drug screening.Advanced healthcare materials · 2014Article
- Real-time cell viability assays using a new anthracycline derivative DRAQ7®.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Cytometry of apoptosis. Historical perspective and new advances.Experimental oncology · 2012Review
- Single molecule fluorescence correlation spectroscopy of single apoptotic cells using a red-fluorescent caspase probe.The Analyst · 2012Article
- The microfluidic system for studies of carcinoma and normal cells interactions after photodynamic therapy (PDT) procedures.Biomicrofluidics · 2011Article
- Ischemia/reperfusion injury of primary porcine cardiomyocytes in a low-shear microfluidic culture and analysis device.The Analyst · 2011Article
- Apoptosis goes on a chip: advances in the microfluidic analysis of programmed cell death.Analytical chemistry · 2011Article
- Real-time cytotoxicity assays.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Rise of the micromachines: microfluidics and the future of cytometry.Methods in cell biology · 2011Article
- Microfluidics: Emerging prospects for anti-cancer drug screening.World journal of clinical oncology · 2010Article
- Bcl-2 inhibits apoptosis by increasing the time-to-death and intrinsic cell-to-cell variations in the mitochondrial pathway of cell death.Apoptosis : an international journal on programmed cell death · 2010Article
- Cell death goes LIVE: technological advances in real-time tracking of cell death.Cell cycle (Georgetown, Tex.) · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Cell cytotoxicity tests are among the most common bioassays using flow cytometry and fluorescence imaging analysis. The permeability of plasma membranes to charged fluorescent probes serves, in these assays, as a marker distinguishing live from dead cells. Since it is generally assumed that probes, such as propidium iodide (PI) or 7-amino-actinomycin D (7-AAD), are themselves cytotoxic, they are currently generally used only as the end-point markers of assays for live versus dead cells. In the current study, we provide novel insights into potential applications of these classical plasma membrane integrity markers in the dynamic tracking of drug-induced cytotoxicity. We show that treatment of a number of different human tumor cell lines in cultures for up to 72 h with the PI, 7-AAD, SYTOX Green (SY-G), SYTOX Red (SY-R), TO-PRO, and YO-PRO had no effect on cell viability assessed by the integrity of plasma membrane, cell cycle progression, and rate of proliferation. We subsequently explore the potential of dynamic labeling with these markers in real-time analysis, by comparing results from both conventional cytometry and microfluidic chips. Considering the simplicity of the staining protocols and their low cost combined with the potential for real-time data collection, we show how that real-time fluorescent imaging and Lab-on-a-Chip platforms have the potential to be used for automated drug screening routines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.