Evidence map›Paper›PMID 19572213›Full record

ArticleMolecular biology reports2010

Screening of genes induced by salt stress from Alfalfa.

Houcong Jin, Yan Sun, Qingchuan Yang, Yuehui Chao, Junmei Kang, Hong Jin, Yan Li, Gruber Margaret

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In one paragraph

Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
10.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 82 citations in OpenAlex.

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  13. Overexpression of a Plasma Membrane-LocalizedFrontiers in plant science · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Houcong JinInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193, Beijing, People's Republic of China.
Yan Sun
Qingchuan Yang
Yuehui Chao
Junmei Kang
Hong Jin
Yan Li
Gruber Margaret
Institute of Animal Sciences · CNBeijing University of Agriculture · CNChinese Academy of Agricultural Sciences · CNSaskatoon Research and Development Centre

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An alfalfa cDNA library induced by salt stress was constructed by suppression subtraction hybridization (SSH) technology. Total RNA from 10-day-old seedlings was used as a "driver," and total RNA from seedlings induced by salt was used as a "tester". One hundred and nineteen clones identified as positive clones by reverse Northern dot-blotting resulted in 82 uni-ESTs comprised of 16 contigs and 66 singletons. Blast analysis of deduced protein sequences revealed that 51 ESTs had identity similar to proteins with known function, while 24 could not be annotated at all. Most of the annotated sequences were homologous to genes involved in abiotic or biotic stress in plants. Among these proteins, beta-amylase, fructose-1,6-bisphosphate, aldolase, and sucrose synthase are related to osmolyte synthesis; a CCCH-type zinc finger protein, DNA binding protein, His-Asp phosphotransfer protein, and the RelA/SpoT protein partake in transcription regulation and signal transduction; and ribulose-l,5-bisphosphate carboxylase/oxygenase, chlorophyll a/b binding proteins, and an early light-inducible proteins are related to photosynthesis. In addition, several ESTs, similar to genes from other plant species, closely involved in salt stress were isolated from alfalfa, such as an aquaporin protein, a late embryogenesis-abundant protein, and glutathione peroxidase.

Indexed as

Genes, PlantDNA, ComplementaryGene Expression Regulation, PlantGene LibraryGenetic TechniquesMedicago sativaReverse Transcriptase Polymerase Chain ReactionSaltsSalt ToleranceStress, PhysiologicalUp-RegulationDNA, ComplementarySalts

Identifiers

PMID19572213
OpenAlexW1971967138

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.