ArticlePloS one2009
dRecQ4 is required for DNA synthesis and essential for cell proliferation in Drosophila.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- RECQL4 is not critical for firing of human DNA replication origins.Scientific reports · 2024Article
- Mitochondrial DNA maintenance in Drosophila melanogaster.Bioscience reports · 2022Review
- Review
- Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells.Oncotarget · 2015Article
- Evolutionary conservation of the CDK targets in eukaryotic DNA replication initiation.Chromosoma · 2015Review
- CDK phosphorylation of SLD-2 is required for replication initiation and germline development in C. elegans.The Journal of cell biology · 2014Article
- Human RecQ helicases in DNA repair, recombination, and replication.Annual review of biochemistry · 2014Review
- CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development.Development (Cambridge, England) · 2013Article
- The Drosophila F-box protein dSkp2 regulates cell proliferation by targeting Dacapo for degradation.Molecular biology of the cell · 2013Article
- The helicase and ATPase activities of RECQL4 are compromised by mutations reported in three human patients.Aging · 2012Article
- Uif, a large transmembrane protein with EGF-like repeats, can antagonize Notch signaling in Drosophila.PloS one · 2012Article
- Drosophila RecQ4 is directly involved in both DNA replication and the response to UV damage in S2 cells.PloS one · 2012Article
- Human RecQL4 helicase plays critical roles in prostate carcinogenesis.Cancer research · 2010Article
- Conserved helicase domain of human RecQ4 is required for strand annealing-independent DNA unwinding.DNA repair · 2010Article
- RecQ4: the second replicative helicase?Critical reviews in biochemistry and molecular biology · 2010Review
- Drosophila RecQ5 is required for efficient SSA repair and suppression of LOH in vivo.Protein & cell · 2010Article
- The role of RecQ helicases in non-homologous end-joining.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe family of RecQ DNA helicases plays an important role in the maintenance of genomic integrity. Mutations in three of the five known RecQ family members in humans, BLM, WRN and RecQ4, lead to disorders that are characterized by predisposition to cancer and premature aging. METHODOLOGY/PRINCIPAL
findingsTo address the in vivo functions of Drosophila RecQ4 (dRecQ4), we generated mutant alleles of dRecQ4 using the targeted gene knock-out technique. Our data show that dRecQ4 mutants are homozygous lethal with defects in DNA replication, cell cycle progression and cell proliferation. Two sets of experiments suggest that dRecQ4 also plays a role in DNA double strand break repair. First, mutant animals exhibit sensitivity to gamma irradiation. Second, the efficiency of DsRed reconstitution via single strand annealing repair is significantly reduced in the dRecQ4 mutant animals. Rescue experiments further show that both the N-terminal domain and the helicase domain are essential to dRecQ4 function in vivo. The N-terminal domain is sufficient for the DNA repair function of dRecQ4. CONCLUSIONS/SIGNIFICANCE: Together, our results show that dRecQ4 is an essential gene that plays an important role in not only DNA replication but also DNA repair and cell cycle progression in vivo.
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