ArticlePhysical biology2009
The temperature dependence of cell mechanics measured by atomic force microscopy.
Article in Physical biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 78 citations in OpenAlex.
- Review
- Indenting multicellular spheroids with various tip geometries.European biophysics journal : EBJ · 2026Article
- Cancer cells metastasize in several types of cancer via two distinct routes, probably based on mechanical signals.Frontiers in cell and developmental biology · 2026Review
- DNA Methylation Carries Signatures of Sublethal Effects Under Thermal Stress in Loggerhead Sea Turtles.Evolutionary applications · 2024Article
- Acoustic Wave-Induced Stroboscopic Optical Mechanotyping of Adherent Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Sounding a New Era in Biomechanics with Acoustic Force Spectroscopy.Advances in experimental medicine and biology · 2023Article
- Measuring the linear viscoelastic regime of MCF-7 cells with a monolayer rheometer in the presence of microtubule-active anti-cancer drugs at high concentrations.Interface focus · 2022Article
- Mechanical properties of cell sheets and spheroids: the link between single cells and complex tissues.Biophysical reviews · 2021Review
- Cell mechanical properties of human breast carcinoma cells depend on temperature.Scientific reports · 2021Article
- Viscoelasticity and Volume of Cortical Neurons under Glutamate Excitotoxicity and Osmotic Challenges.Biophysical journal · 2020Article
- Mapping cellular-scale internal mechanics in 3D tissues with thermally responsive hydrogel probes.Nature communications · 2020Article
- Aqueous Humor Outflow Requires Active Cellular Metabolism in Mice.Investigative ophthalmology & visual science · 2020Article
- High-speed force spectroscopy: microsecond force measurements using ultrashort cantilevers.Biophysical reviews · 2019Review
- Variations of Elastic Modulus and Cell Volume with Temperature for Cortical Neurons.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Experimental research on the relationship between the stiffness and the expressions of fibronectin proteins and adaptor proteins of rat trabecular meshwork cells.BMC ophthalmology · 2017Article
- Directional bleb formation in spherical cells under temperature gradient.Biophysical journal · 2015Article
- Force fluctuations in three-dimensional suspended fibroblasts.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- AFM single-cell force spectroscopy links altered nuclear and cytoskeletal mechanics to defective cell adhesion in cardiac myocytes with a nuclear lamin mutation.Nucleus (Austin, Tex.) · 2015Article
- Impact of heating on passive and active biomechanics of suspended cells.Interface focus · 2014Article
- Biomechanics and the thermotolerance of development.PloS one · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The cytoskeleton is a complex polymer network that regulates the structural stability of living cells. Although the cytoskeleton plays a key role in many important cell functions, the mechanisms that regulate its mechanical behaviour are poorly understood. Potential mechanisms include the entropic elasticity of cytoskeletal filaments, glassy-like inelastic rearrangements of cross-linking proteins and the activity of contractile molecular motors that sets the tensional stress (prestress) borne by the cytoskeleton filaments. The contribution of these mechanisms can be assessed by studying how cell mechanics depends on temperature. The aim of this work was to elucidate the effect of temperature on cell mechanics using atomic force microscopy. We measured the complex shear modulus (G*) of human alveolar epithelial cells over a wide frequency range (0.1-25.6 Hz) at different temperatures (13-37 degrees C). In addition, we probed cell prestress by mapping the contractile forces that cells exert on the substrate by means of traction microscopy. To assess the role of actomyosin contraction in the temperature-induced changes in G* and cell prestress, we inhibited the Rho kinase pathway of the myosin light chain phosphorylation with Y-27632. Our results show that with increasing temperature, cells become stiffer and more solid-like. Cell prestress also increases with temperature. Inhibiting actomyosin contraction attenuated the temperature dependence of G* and prestress. We conclude that the dependence of cell mechanics with temperature is dominated by the contractile activity of molecular motors.
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