ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2009
Identification of dynein light chain road block-1 as a novel interaction partner with the human reduced folate carrier.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Calsyntenin-3 interacts with the sodium-dependent vitamin C transporter-2 to regulate vitamin C uptake.International journal of biological macromolecules · 2021Article
- Identification of transmembrane protein 237 as a novel interactor with the intestinal riboflavin transporter-3 (RFVT-3): role in functionality and cell biology.American journal of physiology. Cell physiology · 2019Article
- N-acetyl-D-glucosamine kinase interacts with dynein light-chain roadblock type 1 at Golgi outposts in neuronal dendritic branch points.Experimental & molecular medicine · 2015Article
- Association of TM4SF4 with the human thiamine transporter-2 in intestinal epithelial cells.Digestive diseases and sciences · 2014Article
- Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- Requirement for protein kinase A in the phosphorylation of the TGFβ receptor-interacting protein km23-1 as a component of TGFβ downstream effects.Experimental cell research · 2013Article
- Molecular cloning of the Robl gene from Bombyx mori and studies of its developmental and physicochemical regulation.Molecular biology reports · 2012Article
- Tspan-1 interacts with the thiamine transporter-1 in human intestinal epithelial cells and modulates its stability.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Intestinal absorption of water-soluble vitamins in health and disease.The Biochemical journal · 2011Review
- Association of PDZ-containing protein PDZD11 with the human sodium-dependent multivitamin transporter.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Light chain-dependent self-association of dynein intermediate chain.The Journal of biological chemistry · 2011Article
- Structure-function activity of the human sodium-dependent multivitamin transporter: role of His¹¹⁵ and His²⁵⁴.American journal of physiology. Cell physiology · 2011Article
- The crystal structure of dynein intermediate chain-light chain roadblock complex gives new insights into dynein assembly.The Journal of biological chemistry · 2010Article
- Molecular determinants dictating cell surface expression of the human sodium-dependent vitamin C transporter-2 in human liver cells.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The reduced folate carrier (RFC) is a major folate transport system in mammalian cells. RFC is highly expressed in the intestine and believed to play a role in folate absorption. Studies from our laboratory and others have characterized different aspects of the intestinal folate absorption process, but little is known about possible existence of accessory protein(s) that interacts with RFC and influences its physiology and/or cell biology. We investigated this issue by employing a bacterial two-hybrid system to screen a BacterioMatch II human intestinal cDNA library using the large intracellular loop between transmembrane domains 6 and 7 of the human RFC (hRFC) as bait. Our screening has resulted in the identification of dynein light chain road block-1 (DYNLRB1) as an interacting partner with hRFC. Existence of a direct protein-protein interaction between hRFC and DYNLRB1 was confirmed by in vitro pull-down assay and in vivo mammalian two-hybrid luciferase assay and coimmunoprecipitation analysis. Furthermore, confocal imaging of live human intestinal epithelial HuTu-80 cells demonstrated colocalization of DYNLRB1 with hRFC. Coexpression of DYNLRB1 with hRFC led to a significant (P < 0.05) increase in folate uptake. On the other hand, inhibiting the endogenous DYNLRB1 with gene-specific small interfering RNA or pharmacologically with a specific inhibitor (vanadate) led to a significant (P < 0.05) decrease in folate uptake. This study demonstrates for the first time the identification of DYNLRB1 as an interacting protein partner with hRFC. Furthermore, DYNLRB1 appears to influence the function and cell biology of hRFC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.