ArticleClinical and vaccine immunology : CVI2009
Optimization of a whole-blood gamma interferon assay for detection of Mycobacterium bovis-infected cattle.
Article in Clinical and vaccine immunology : CVI, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Exploring specific biomarkers in blood forFrontiers in microbiology · 2026Article
- Comparative Performance of Ante-Mortem Diagnostic Assays for the Identification ofPathogens (Basel, Switzerland) · 2025Article
- Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis.Scientific reports · 2024Article
- Evaluation of the performance of the IFN-γ release assay in bovine tuberculosis free herds from five European countries.Veterinary research · 2023Article
- Efficacy of SAT2 Foot-and-Mouth Disease Vaccines Formulated with Montanide ISA 206B and Quil-A Saponin Adjuvants.Vaccines · 2021Article
- Serial Interferon-Gamma Release Assay (IGRA) Testing to Monitor Treatment Responses in Cases of Feline Mycobacteriosis.Pathogens (Basel, Switzerland) · 2021Article
- Evaluation of Three Commercial Interferon-γ Assays in a Bovine Tuberculosis Free Population.Frontiers in veterinary science · 2021Article
- Article
- Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo (Frontiers in veterinary science · 2020Article
- Detection of native interferon-γ in nyala (Tragelaphus angasii): Towards diagnosing tuberculosis.The Onderstepoort journal of veterinary research · 2019Article
- An outbreak of tuberculosis due to Mycobacterium bovis infection in a pack of English Foxhounds (2016-2017).Transboundary and emerging diseases · 2018Article
- Successful Application of the Gamma-Interferon Assay in a Bovine Tuberculosis Eradication Program: The French Bullfighting Herd Experience.Frontiers in veterinary science · 2018Article
- Development and evaluation of an interferon gamma assay for the diagnosis of tuberculosis in red deer experimentally infected with Mycobacterium bovis.BMC veterinary research · 2017Article
- Evaluating diagnostic tests for bovine tuberculosis in the southern part of Germany: A latent class analysis.PloS one · 2017Article
- Stimulation of Bovine Whole-Blood Samples Cultured in Media Supplemented with Recombinant Interleukin-7 (IL-7) and IL-12 Extends the Life Span of the Gamma Interferon Assay To Detect Mycobacterium bovis-Infected Cattle.Journal of clinical microbiology · 2016Article
- Evaluation of ethanol vortex ELISA for detection of bovine tuberculosis in cattle and deer.BMC veterinary research · 2014Article
- Clinical and diagnostic developments of a gamma interferon release assay for use in bovine tuberculosis control programs.Clinical and vaccine immunology : CVI · 2013Article
- A tutorial in estimating the prevalence of disease in humans and animals in the absence of a gold standard diagnostic.Emerging themes in epidemiology · 2012Article
- Evaluation of gamma interferon and antibody tuberculosis tests in alpacas.Clinical and vaccine immunology : CVI · 2012Article
- Incubation of whole blood at 39°C augments gamma interferon (IFN-γ)-induced protein 10 and IFN-γ responses to Mycobacterium tuberculosis antigens.Clinical and vaccine immunology : CVI · 2011Article
Corrections and comments
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Authors and funding
15 authors.
Funding
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Abstract
Antigens of Mycobacterium bovis elicit a cell-mediated immune response upon intradermal injection in cattle. In vitro, such antigens stimulate the production of gamma interferon (IFN-gamma) by bovine T cells in whole-blood culture (IFN-gamma assay). We have analyzed various parameters of the in vitro IFN-gamma assay, ranging from blood sampling to execution of the IFN-gamma test, in view of potential simplifications of the assay. Here, we show that IFN-gamma responses may be reduced under certain animal handling/holding conditions and that a delayed time from blood collection to culture may lead to a reduced in vitro IFN-gamma response. Delayed initiation of culture in a purified-protein-derivative-based assay (24 h compared to 8 h after blood collection), however, resulted in a significant improvement of specificity (97% compared to 85%), whereas there was only a modest reduction of sensitivity (from 96% to 90%), which was statistically not significant. Furthermore, we show that the stimulation temperature needs to be 33 degrees C or higher; that carbon dioxide is not required for stimulation; and that various plate formats, ranging from 24 to 96 wells per plate, can be utilized. The produced IFN-gamma is stable at 4 degrees C for 28 days as well as after repeated freeze-thaw cycles. Thus, stimulation of samples may be initiated in the field without the need for a carbon dioxide source, and bovine IFN-gamma is stable under various routine laboratory temperature scenarios. These findings demonstrate opportunities for improvements in the bovine IFN-gamma test platform and flexibilities in test application.
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