ArticleMolecular biology of the cell2009
Drosophila GoLoco-protein Pins is a target of Galpha(o)-mediated G protein-coupled receptor signaling.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Rapid purification of brain protein complexes containing active and inactive forms of the G protein Gαo.PloS one · 2025Article
- Neomorphic Gαo mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies.The Journal of clinical investigation · 2024Article
- Article
- Restoration of the GTPase activity and cellular interactions of GαScience advances · 2022Article
- Pediatric Encephalopathy: Clinical, Biochemical and Cellular Insights into the Role of Gln52 ofCells · 2021Article
- Humanization ofBiomedicines · 2020Article
- Diversity of activator of G-protein signaling (AGS)-family proteins and their impact on asymmetric cell division across taxa.Developmental biology · 2020Review
- Article
- Antagonistic PCP Signaling Pathways in the developing Drosophila eye.Scientific reports · 2018Article
- WNT signalling events near the cell membrane and their pharmacological targeting for the treatment of cancer.British journal of pharmacology · 2017Review
- Meru couples planar cell polarity with apical-basal polarity during asymmetric cell division.eLife · 2017Article
- Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine nucleotides.Bioscience reports · 2015Article
- Isoform-specific functions of Mud/NuMA mediate binucleation of Drosophila male accessory gland cells.BMC developmental biology · 2014Article
- Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton.Development (Cambridge, England) · 2014Article
- Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster.The European journal of neuroscience · 2014Article
- Activators of G protein signaling exhibit broad functionality and define a distinct core signaling triad.Molecular pharmacology · 2014Review
- Regulation of the G-protein regulatory-Gαi signaling complex by nonreceptor guanine nucleotide exchange factors.The Journal of biological chemistry · 2013Article
- Kermit interacts with Gαo, Vang, and motor proteins in Drosophila planar cell polarity.PloS one · 2013Article
- Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster Gαo-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Crystal structures of the scaffolding protein LGN reveal the general mechanism by which GoLoco binding motifs inhibit the release of GDP from Gαi.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G protein-coupled receptors (GPCRs) transduce their signals through trimeric G proteins, inducing guanine nucleotide exchange on their Galpha-subunits; the resulting Galpha-GTP transmits the signal further inside the cell. GoLoco domains present in many proteins play important roles in multiple trimeric G protein-dependent activities, physically binding Galpha-subunits of the Galpha(i/o) class. In most cases GoLoco binds exclusively to the GDP-loaded form of the Galpha-subunits. Here we demonstrate that the poly-GoLoco-containing protein Pins of Drosophila can bind to both GDP- and GTP-forms of Drosophila Galpha(o). We identify Pins GoLoco domain 1 as necessary and sufficient for this unusual interaction with Galpha(o)-GTP. We further pinpoint a lysine residue located centrally in this domain as necessary for the interaction. Our studies thus identify Drosophila Pins as a target of Galpha(o)-mediated GPCR receptor signaling, e.g., in the context of the nervous system development, where Galpha(o) acts downstream from Frizzled and redundantly with Galpha(i) to control the asymmetry of cell divisions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.