ArticleMolecular biology of the cell2009
Distinct roles for key karyogamy proteins during yeast nuclear fusion.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Revolutionizing molecular plant breeding through genetic engineering of in vivo haploid induction genes.Molecular biology reports · 2025Review
- Nuclear Fusion in Yeast and Plant Reproduction.Plants (Basel, Switzerland) · 2023Article
- PRM1 and KAR5 function in cell-cell fusion and karyogamy to drive distinct bisexual and unisexual cycles in the Cryptococcus pathogenic species complex.PLoS genetics · 2017Article
- Genetic Evidence for Sexuality in Cochliopodium (Amoebozoa).The Journal of heredity · 2017Article
- Influence of the bud neck on nuclear envelope fission in Saccharomyces cerevisiae.Experimental cell research · 2017Article
- The Malleable Nature of the Budding Yeast Nuclear Envelope: Flares, Fusion, and Fenestrations.Journal of cellular physiology · 2016Review
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Kar5p is required for multiple functions in both inner and outer nuclear envelope fusion in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2014Article
- Absence of SUN-domain protein Slp1 blocks karyogamy and switches meiotic recombination and synapsis from homologs to sister chromatids.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Dynamics of Male and Female Chromatin during Karyogamy in Rice Zygotes.Plant physiology · 2014Article
- Breaching the nuclear envelope in development and disease.The Journal of cell biology · 2014Review
- ER-associated SNAREs and Sey1p mediate nuclear fusion at two distinct steps during yeast mating.Molecular biology of the cell · 2013Article
- Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.Genes & development · 2013Article
- Dynamic assembly of brambleberry mediates nuclear envelope fusion during early development.Cell · 2012Article
- The dynamin-like GTPase Sey1p mediates homotypic ER fusion in S. cerevisiae.The Journal of cell biology · 2012Article
- Conservation of proteo-lipid nuclear membrane fusion machinery during early embryogenesis.Nucleus (Austin, Tex.)Article
- Cytosol-dependent membrane fusion in ER, nuclear envelope and nuclear pore assembly: biological implications.Nucleus (Austin, Tex.)Article
- When yeast cells meet, karyogamy!: an example of nuclear migration slowly resolved.Nucleus (Austin, Tex.)Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
During yeast mating, cell fusion is followed by the congression and fusion of the two nuclei. Proteins required for nuclear fusion are found at the surface (Prm3p) and within the lumen (Kar2p, Kar5p, and Kar8p) of the nuclear envelope (NE). Electron tomography (ET) of zygotes revealed that mutations in these proteins block nuclear fusion with different morphologies, suggesting that they act in different steps of fusion. Specifically, prm3 zygotes were blocked before formation of membrane bridges, whereas kar2, kar5, and kar8 zygotes frequently contained them. Membrane bridges were significantly larger and occurred more frequently in kar2 and kar8, than in kar5 mutant zygotes. The kinetics of NE fusion in prm3, kar5, and kar8 mutants, measured by live-cell fluorescence microscopy, were well correlated with the size and frequency of bridges observed by ET. However the kar2 mutant was defective for transfer of NE lumenal GFP, but not diffusion within the lumen, suggesting that transfer was blocked at the NE fusion junction. These observations suggest that Prm3p acts before initiation of outer NE fusion, Kar5p may help dilation of the initial fusion pore, and Kar2p and Kar8p act after outer NE fusion, during inner NE fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.