ArticleMolecular biology of the cell2009
Regulation of fission yeast myosin-II function and contractile ring dynamics by regulatory light-chain and heavy-chain phosphorylation.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Effects ofCurrent issues in molecular biology · 2024Article
- Article
- Myosin turnover controls actomyosin contractile instability.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast.eLife · 2020Article
- A Cdc42 GEF, Gef1, through endocytosis organizes F-BAR Cdc15 along the actomyosin ring and promotes concentric furrowing.Journal of cell science · 2019Article
- Mechanisms of contractile ring tension production and constriction.Biophysical reviews · 2018Review
- Fission yeast Myo2: Molecular organization and diffusion in the cytoplasm.Cytoskeleton (Hoboken, N.J.) · 2018Article
- A node organization in the actomyosin contractile ring generates tension and aids stability.Molecular biology of the cell · 2017Article
- Fission yeast myosin Myo2 is down-regulated in actin affinity by light chain phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis.Molecular biology of the cell · 2015Article
- Characterization of the roles of Blt1p in fission yeast cytokinesis.Molecular biology of the cell · 2014Article
- UCS protein Rng3p is essential for myosin-II motor activity during cytokinesis in fission yeast.PloS one · 2013Article
- Contractile-ring assembly in fission yeast cytokinesis: Recent advances and new perspectives.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis.Molecular biology of the cell · 2012Article
- Functional adaptation between yeast actin and its cognate myosin motors.The Journal of biological chemistry · 2011Article
- On the cutting edge: post-translational modifications in cytokinesis.Trends in cell biology · 2011Review
- Three's company: the fission yeast actin cytoskeleton.Trends in cell biology · 2011Review
- Dividing the spoils of growth and the cell cycle: The fission yeast as a model for the study of cytokinesis.Cytoskeleton (Hoboken, N.J.) · 2011Review
- Mechanisms of contractile-ring assembly in fission yeast and beyond.Seminars in cell & developmental biology · 2010Article
- Tropomyosin and myosin-II cellular levels promote actomyosin ring assembly in fission yeast.Molecular biology of the cell · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the role of regulatory light-chain (Rlc1p) and heavy-chain phosphorylation in controlling fission yeast myosin-II (Myo2p) motor activity and function during cytokinesis. Phosphorylation of Rlc1p leads to a fourfold increase in Myo2p's in vitro motility rate, which ensures effective contractile ring constriction and function. Surprisingly, unlike with smooth muscle and nonmuscle myosin-II, RLC phosphorylation does not influence the actin-activated ATPase activity of Myo2p. A truncated form of Rlc1p lacking its extended N-terminal regulatory region (including phosphorylation sites) supported maximal Myo2p in vitro motility rates and normal contractile ring function. Thus, the unphosphorylated N-terminal extension of Rlc1p can uncouple the ATPase and motility activities of Myo2p. We confirmed the identity of one out of two putative heavy-chain phosphorylation sites previously reported to control Myo2p function and cytokinesis. Although in vitro studies indicated that phosphorylation at Ser-1444 is not needed for Myo2p motor activity, phosphorylation at this site promotes the initiation of contractile ring constriction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.