ArticleMolecular pain2009
Synaptically evoked glutamate transporter currents in Spinal Dorsal Horn Astrocytes.
Article in Molecular pain, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Spinal neuron-glial crosstalk and ion channel dysregulation in diabetic neuropathic pain.Frontiers in immunology · 2025Review
- Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment.The Journal of comparative neurology · 2016Article
- Targeted Overexpression of Astrocytic Endothelin-1 Attenuates Neuropathic Pain by Upregulating Spinal Excitatory Amino Acid Transporter-2.Journal of molecular neuroscience : MN · 2015Article
- Paclitaxel induces acute pain via directly activating toll like receptor 4.Molecular pain · 2015Article
- Activation of toll like receptor 4 attenuates GABA synthesis and postsynaptic GABA receptor activities in the spinal dorsal horn via releasing interleukin-1 beta.Journal of neuroinflammation · 2015Article
- Plasticity of Neuron-Glial Transmission: Equipping Glia for Long-Term Integration of Network Activity.Neural plasticity · 2015Review
- Astrocytes, but not microglia, are activated in oxaliplatin and bortezomib-induced peripheral neuropathy in the rat.Neuroscience · 2014Article
- Article
- How do astrocytes shape synaptic transmission? Insights from electrophysiology.Frontiers in cellular neuroscience · 2013Review
- Spinal astrocyte gap junctions contribute to oxaliplatin-induced mechanical hypersensitivity.The journal of pain · 2013Article
- Whiplash-like facet joint loading initiates glutamatergic responses in the DRG and spinal cord associated with behavioral hypersensitivity.Brain research · 2012Article
- Evidence that spinal astrocytes but not microglia contribute to the pathogenesis of Paclitaxel-induced painful neuropathy.The journal of pain · 2012Article
- Acute inhibition of signalling phenotype of spinal GABAergic neurons by tumour necrosis factor-alpha.The Journal of physiology · 2011Article
- Cancer-induced bone pain sequentially activates the ERK/MAPK pathway in different cell types in the rat spinal cord.Molecular pain · 2011Article
- The effect of spider toxin PhTx3-4, ω-conotoxins MVIIA and MVIIC on glutamate uptake and on capsaicin-induced glutamate release and [Ca2+]i in spinal cord synaptosomes.Cellular and molecular neurobiology · 2011Article
- A p38 mitogen-activated protein kinase-dependent mechanism of disinhibition in spinal synaptic transmission induced by tumor necrosis factor-alpha.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Spinal upregulation of glutamate transporter GLT-1 by ceftriaxone: therapeutic efficacy in a range of experimental nervous system disorders.Neuroscience · 2010Article
- Bidirectional neuron-glia interactions triggered by deficiency of glutamate uptake at spinal sensory synapses.Journal of neurophysiology · 2010Article
- Impaired glial glutamate uptake induces extrasynaptic glutamate spillover in the spinal sensory synapses of neuropathic rats.Journal of neurophysiology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundRemoving and sequestering synaptically released glutamate from the extracellular space is carried out by specific plasma membrane transporters that are primarily located in astrocytes. Glial glutamate transporter function can be monitored by recording the currents that are produced by co-transportation of Na+ ions with the uptake of glutamate. The goal of this study was to characterize glutamate transporter function in astrocytes of the spinal cord dorsal horn in real time by recording synaptically evoked glutamate transporter currents.
resultsWhole-cell patch clamp recordings were obtained from astrocytes in the spinal substantia gelatinosa (SG) area in spinal slices of young adult rats. Glutamate transporter currents were evoked in these cells by electrical stimulation at the spinal dorsal root entry zone in the presence of bicuculline, strychnine, DNQX and D-AP5. Transporter currents were abolished when synaptic transmission was blocked by TTX or Cd2+. Pharmacological studies identified two subtypes of glutamate transporters in spinal astrocytes, GLAST and GLT-1. Glutamate transporter currents were graded with stimulus intensity, reaching peak responses at 4 to 5 times activation threshold, but were reduced following low-frequency (0.1 - 1 Hz) repetitive stimulation.
conclusionThese results suggest that glutamate transporters of spinal astrocytes could be activated by synaptic activation, and recording glutamate transporter currents may provide a means of examining the real time physiological responses of glial cells in spinal sensory processing, sensitization, hyperalgesia and chronic pain.
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