ArticleBMC microbiology2009
Simultaneous differential detection of Chlamydophila abortus, Chlamydophila pecorum and Coxiella burnetii from aborted ruminant's clinical samples using multiplex PCR.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Current status and future directions ofFrontiers in veterinary science · 2026Pooled it
- Epidemiology of Coxiella burnetii infection in Africa: a OneHealth systematic review.PLoS neglected tropical diseases · 2014Pooled it
- Tick-Borne Bacteria and Protozoa inBiology · 2026Article
- Molecular detection of Chlamydia psittaci in wild birds, broiler chickens, and sheep in Egypt: a public health concern.BMC veterinary research · 2026Article
- A Note on Some Health-Related Outcomes in Small Ruminant Farms with Common Grazing with Wildlife Ruminants.Animals : an open access journal from MDPI · 2025Article
- Hemotropic pathogens in aborted fetuses of domestic ruminants: transplacental transmission and implications for reproductive loss.Frontiers in microbiology · 2025Article
- Review
- Survey on the Occurrence of Zoonotic Bacterial Pathogens in the Feces of Wolves (Microorganisms · 2024Article
- Molecular Investigation of Small Ruminant Abortions Using a 10-Plex HRM-qPCR Technique: A Novel Approach in Routine Diagnostics.Microorganisms · 2024Article
- Molecular Survey on the Occurrence of Tick-Borne Bacteria in Wild Birds from Central Italy.Veterinary sciences · 2024Article
- Antibody-Based Assessment ofAnimals : an open access journal from MDPI · 2023Article
- Human-biting ticks and zoonotic tick-borne pathogens in North Africa: diversity, distribution, and trans-Mediterranean public health challenges.One health (Amsterdam, Netherlands) · 2023Review
- Seroprevalence of zoonotic abortive diseases and their associated risk factors in Tunisian sheep.BMC veterinary research · 2023Article
- Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion.Pathogens (Basel, Switzerland) · 2023Article
- Coxiellosis in Livestock: Epidemiology, Public Health Significance, and Prevalence ofVeterinary medicine (Auckland, N.Z.) · 2023Review
- Infectious abortions in small domestic ruminants in the Iberian Peninsula: Optimization of sampling procedures for molecular diagnostics.Frontiers in veterinary science · 2023Article
- Article
- Retrospective Molecular Survey on Bacterial and Protozoan Abortive Agents in Roe Deer (Capreolus capreolus) from Central ItalyAnimals : an open access journal from MDPI · 2022Article
- Occurrence of Bacterial and Protozoan Pathogens in Red Foxes (Animals : an open access journal from MDPI · 2022Article
- Genetic diversity of Chlamydia pecorum detected in sheep flocks from Mexico.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChlamydiosis and Q fever, two zoonosis, are important causes of ruminants' abortion around the world. They are caused respectively by strictly intracellular and Gram negative bacterium Chlamydophila abortus (Cp. abortus) and Coxiella burnetii (C. burnetii). Chlamydophila pecorum (Cp. pecorum) is commonly isolated from the digestive tract of clinically inconspicuous ruminants but the abortive and zoonotic impact of this bacterium is still unknown because Cp. pecorum is rarely suspected in abortion cases of small ruminants. We have developed a multiplex PCR (m-PCR) for rapid simultaneous differential detection of Cp. abortus, Cp. pecorum and C. burnetii in clinical samples taken from infected animals.
resultsSpecific PCR primers were designed and a sensitive and specific m-PCR was developed to detect simultaneously, in one tube reaction, three specific fragments of 821, 526 and 687-bp long for Cp. abortus, Cp. pecorum and C. burnetii respectively. This m-PCR assay was performed on 253 clinical samples taken from infected ruminant's flocks that have showed problems of abortion diseases. Thus, 67 samples were infected by either one of the three pathogens: 16 (13 vaginal swabs and 3 placentas) were positive for Cp. abortus, 2 were positive for Cp. pecorum (1 vaginal swab and 1 placenta) and 49 samples (33 vaginal swabs, 11 raw milks, 4 faeces and 1 placenta) were positive for C. burnetii. Two vaginal swabs were m-PCR positive of both Cp. abortus and C. burnetii and none of the tested samples was shown to be infected simultaneously with the three pathogens.
conclusionWe have successfully developed a rapid multiplex PCR that can detect and differentiate Cp. abortus, Cp. pecorum and C. burnetii; with a good sensitivity and specificity. The diagnosis of chlamydiosis and Q fever may be greatly simplified and performed at low cost. In addition, the improvement in diagnostic techniques will enhance our knowledge regarding the prevalence and the pathogenetic significance of Q fever and chlamydiosis.
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