ArticleASN neuro2009
Point mutation in the mouse P2X7 receptor affects intercellular calcium waves in astrocytes.
Article in ASN neuro, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Src family kinases activity is required for transmitting purinergic P2X7 receptor signaling in cortical spreading depression and neuroinflammation.The journal of headache and pain · 2021Article
- Article
- The P2X7 ion channel is dispensable for energy and metabolic homeostasis of white and brown adipose tissues.Purinergic signalling · 2020Article
- P2X7 Receptor is Involved in Mitochondrial Dysfunction Induced by Extracellular Alpha Synuclein in Neuroblastoma SH-SY5Y Cells.International journal of molecular sciences · 2020Article
- Role of pannexin 1 channels in load-induced skeletal response.Annals of the New York Academy of Sciences · 2019Article
- Neuronal P2X7 Receptors Revisited: Do They Really Exist?The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Review
- Article
- P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes.PloS one · 2016Article
- Control of the neurovascular coupling by nitric oxide-dependent regulation of astrocytic Ca(2+) signaling.Frontiers in cellular neuroscience · 2015Review
- Functional P2X7 receptors at cultured hippocampal astrocytes but not neurons.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- The membrane protein Pannexin1 forms two open-channel conformations depending on the mode of activation.Science signaling · 2014Article
- Pannexin 1 channels play essential roles in urothelial mechanotransduction and intercellular signaling.PloS one · 2014Article
- P2X7 receptor inhibition increases CNTF in the subventricular zone, but not neurogenesis or neuroprotection after stroke in adult mice.Translational stroke research · 2013Article
- Intercellular Ca(2+) waves: mechanisms and function.Physiological reviews · 2012Review
- Article
- Lipid metabolism modulation by the P2X7 receptor in the immune system and during the course of infection: new insights into the old view.Purinergic signalling · 2011Article
- The role of the P2X₇ receptor in infectious diseases.PLoS pathogens · 2011Review
- C terminus of the P2X7 receptor: treasure hunting.Purinergic signalling · 2011Article
- Article
- Neuroglial ATP release through innexin channels controls microglial cell movement to a nerve injury.The Journal of general physiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Purinergic P2 receptors and gap junctions are two groups of proteins involved in the transmission of ICWs (intercellular calcium waves) between astrocytes. The extent to which ICWs spread among these glial cells depends on the amount of ATP released, which can occur through membrane channels, as well as other pathways. Our previous studies have shown that the pore-forming P2X7R (P2X7 receptor) contributes to the amplification of ICW spread by providing sites of ATP release through Panx1 (Pannexin1) channels. To gain insight into the signal transduction events mediating this response we compared the properties of the P2X7R-Panx1 complex in astrocytes from a mouse strain (C57Bl/6) containing a naturally occurring point mutation (P451L) in the C-terminus of the P2X7R to that of non-mutated receptors (Balb/C mice). Electrophysiological, biochemical, pharmacological and fluorescence imaging techniques revealed that the P451L mutation located in the SH3 domain (a Src tyrosine kinase-binding site) of the C-terminus of the P2X7R attenuates Panx1 currents, ATP release and the distance of ICW spread between astrocytes. Similar results were obtained when using the Src tyrosine inhibitor (PP2) and a membrane-permeant peptide spanning the P451L mutation of the P2X7R of the C57Bl6 astrocytes. These results support the participation of a tyrosine kinase of the Src family in the initial steps mediating the opening of Panx1 channels following P2X7R stimulation and in the transmission of calcium signals among astrocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.