ArticlePloS one2009
Targeted deletion of multiple CTCF-binding elements in the human C-MYC gene reveals a requirement for CTCF in C-MYC expression.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Direction and modality of transcription changes caused by TAD boundary disruption in Slc29a3/Unc5b locus depends on tissue-specific epigenetic context.Epigenetics & chromatin · 2025Article
- Structural insights into a highly flexible zinc finger module unravel INSM1 function in transcription regulation.Nature communications · 2025Article
- Allelic chromatin structure precedes imprinted expression ofGenes & development · 2023Article
- Active enhancers strengthen insulation by RNA-mediated CTCF binding at chromatin domain boundaries.Genome research · 2023Article
- Induced hepatic stem cells maintain self-renewal through the high expression of Myc coregulated by TET1 and CTCF.Cell & bioscience · 2022Article
- Review
- Regulation of host and viral promoters during human cytomegalovirus latency via US28 and CTCF.The Journal of general virology · 2021Article
- NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia.Leukemia · 2020Article
- Article
- Epstein-Barr Virus Rta-Mediated Accumulation of DNA Methylation Interferes with CTCF Binding in both Host and Viral Genomes.Journal of virology · 2017Article
- Mice deficient ofeLife · 2017Article
- ChIA-PET2: a versatile and flexible pipeline for ChIA-PET data analysis.Nucleic acids research · 2017Article
- Systematic mapping of functional enhancer-promoter connections with CRISPR interference.Science (New York, N.Y.) · 2016Article
- BPTF is required for c-MYC transcriptional activity and in vivo tumorigenesis.Nature communications · 2016Article
- The DNA-binding factor Ctcf critically controls gene expression in macrophages.Cellular & molecular immunology · 2014Article
- Genomic imprinting and epigenetic control of development.Cold Spring Harbor perspectives in biology · 2012Article
- Review
- ADP-ribose polymers localized on Ctcf-Parp1-Dnmt1 complex prevent methylation of Ctcf target sites.The Biochemical journal · 2012Article
- Comprehensive identification and annotation of cell type-specific and ubiquitous CTCF-binding sites in the human genome.PloS one · 2012Article
- Characterization of ARF-BP1/HUWE1 interactions with CTCF, MYC, ARF and p53 in MYC-driven B cell neoplasms.International journal of molecular sciences · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundInsulators and domain boundaries both shield genes from adjacent enhancers and inhibit intrusion of heterochromatin into transgenes. Previous studies examined the functional mechanism of the MYC insulator element MINE and its CTCF binding sites in the context of transgenes that were randomly inserted into the genome by transfection. However, the contribution of CTCF binding sites to both gene regulation and maintenance of chromatin has not been tested at the endogenous MYC gene. METHODOLOGY/PRINCIPAL
findingsTo determine the impact of CTCF binding on MYC expression, a series of mutant human chromosomal alleles was prepared in homologous recombination-efficient DT40 cells and individually transferred by microcell fusion into murine cells. Functional tests reported here reveal that deletion of CTCF binding elements within the MINE does not impact the capacity of this locus to correctly organize an 'accessible' open chromatin domain, suggesting that these sites are not essential for the formation of a competent, transcriptionally active locus. Moreover, deletion of the CTCF site at the MYC P2 promoter reduces transcription but does not affect promoter acetylation or serum-inducible transcription. Importantly, removal of either CTCF site leads to DNA methylation of flanking sequences, thereby contributing to progressive loss of transcriptional activity.
conclusionsThese findings collectively demonstrate that CTCF-binding at the human MYC locus does not repress transcriptional activity but is required for protection from DNA methylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.