Evidence map›Paper›PMID 19566682›Full record

ArticleFEMS microbiology letters2009

Tryptophan auxotrophs were obtained by random transposon insertions in the Methanococcus maripaludis tryptophan operon.

Iris Porat, William B Whitman

Abstract readEvaluation Study
In one paragraph

Article in FEMS microbiology letters, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Teaching old dogs new tricks: genetic engineering methanogens.Applied and environmental microbiology · 2024
    Review
  2. Article
  3. Review
  4. A Flexible System for Cultivation ofArchaea (Vancouver, B.C.) · 2017
    Article
  5. Genome-scale analysis of gene function in the hydrogenotrophic methanogenic archaeon Methanococcus maripaludis.Proceedings of the National Academy of Sciences of the United States of America · 2013
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Iris Porat
William B Whitman

Funding

Methanococcus maripaludis as an emerging model organismR24GM074783 · NIGMS · UNIVERSITY OF WASHINGTON · PI LEIGH, JOHN A · 2007 to 2010
$915k
NIGMS NIH HHS R24 GM074783
6 · The paper itself

Abstract

Methanococcus maripaludis is an anaerobic, methane-producing archaeon that utilizes H(2) or formate for the reduction of CO(2) to methane. Tryptophan auxotrophs were constructed by in vitro insertions of the Tn5 transposon into the tryptophan operon, followed by transformation into M. maripaludis. This method could serve for rapid insertions into large cloned DNA regions.

Indexed as

DNA Transposable ElementsOperonAutotrophic ProcessesMethanococcusMutagenesis, InsertionalTryptophanDNA Transposable ElementsTryptophan

Identifiers

PMID19566682
PMCPMC2771393

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.