ArticleJournal of thrombosis and haemostasis : JTH2009
Binding of platelet glycoprotein Ibbeta through the convex surface of leucine-rich repeats domain of glycoprotein IX.
Article in Journal of thrombosis and haemostasis : JTH, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- New insights of glycoprotein Ib-IX-V complex organization and glycoprotein Ibα in platelet biogenesis.Current opinion in hematology · 2024Review
- Establishment of a Bernard-Soulier syndrome model in zebrafish.Haematologica · 2022Article
- Platelets and Defective N-Glycosylation.International journal of molecular sciences · 2020Review
- Defective Association of the Platelet Glycoprotein Ib-IX Complex with the Glycosphingolipid-Enriched Membrane Domain Inhibits Murine Thrombus and Atheroma Formation.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- The Transmembrane Domains of β and IX Subunits Mediate the Localization of the Platelet Glycoprotein Ib-IX Complex to the Glycosphingolipid-enriched Membrane Domain.The Journal of biological chemistry · 2015Article
- Analysis of inter-subunit contacts reveals the structural malleability of extracellular domains in platelet glycoprotein Ib-IX complex.Journal of thrombosis and haemostasis : JTH · 2014Article
- Specific inhibition of ectodomain shedding of glycoprotein Ibα by targeting its juxtamembrane shedding cleavage site.Journal of thrombosis and haemostasis : JTH · 2013Article
- The organizing principle of the platelet glycoprotein Ib-IX-V complex.Journal of thrombosis and haemostasis : JTH · 2013Review
- Transmembrane domains are critical to the interaction between platelet glycoprotein V and glycoprotein Ib-IX complex.Journal of thrombosis and haemostasis : JTH · 2012Article
- Reconstitution of the platelet glycoprotein Ib-IX complex in phospholipid bilayer Nanodiscs.Biochemistry · 2011Article
- Article
- Molecular insight into human platelet antigens: structural and evolutionary conservation analyses offer new perspective to immunogenic disorders.Transfusion · 2011Article
- Article
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Authors and funding
7 authors.
Funding
Abstract
backgroundThe mechanism of assembly of the platelet glycoprotein (GP) Ib-IX complex from GPIbalpha, GPIbbeta and GPIX subunits is not entirely clear. In this complex, ectodomains of both GPIbbeta and GPIX subunits contain two leucine-rich repeats (LRR) and share high sequence similarity. However, they differ noticeably in stability, hampering further analysis of their interaction. OBJECTIVES AND
methodsGuided by analysis of the LRR structure, we report a well-folded Ibbeta/IX chimera and its usage in dissecting GPIX function.
resultsIn this chimera, three non-contiguous sequences that may constitute the putative convex surface of the GPIbbeta ectodomain are replaced by their GPIX counterparts. Like GPIbbeta but unlike GPIX ectodomain, it can secrete from transfected Chinese hamster ovary cells and fold into a stable conformation. Furthermore, replacing the ectodomain in GPIX with the Ibbeta/IX chimera, but not the GPIbbeta ectodomain, preserved its interaction with GPIbbeta as demonstrated by its native-like GPIbbeta-induced increase in surface expression and coimmunoprecipitation.
conclusionsThe putative convex surface of the LRR domain in GPIX is sufficient, in the context of full-length subunit, to mediate its association with GPIbbeta.
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