ArticleJournal of cellular biochemistry2009
Threonine 393 of beta-catenin regulates interaction with Axin.
Article in Journal of cellular biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- CK2 and the Hallmarks of Cancer.Biomedicines · 2022Review
- The Role of Protein Kinase CK2 in Development and Disease Progression: A Critical Review.Journal of developmental biology · 2022Review
- Article
- Phosphorylation-Dependent Regulation of WNT/Beta-Catenin Signaling.Frontiers in oncology · 2022Review
- Protein Kinase CK2 in Cancer Energetics.Frontiers in oncology · 2020Review
- Skin renewal activity of non-thermal plasma through the activation of β-catenin in keratinocytes.Scientific reports · 2017Article
- The γ-Protocadherin-C3 isoform inhibits canonical Wnt signalling by binding to and stabilizing Axin1 at the membrane.Scientific reports · 2016Article
- Protein kinase CK2 in breast cancer: the CK2β regulatory subunit takes center stage in epithelial plasticity.Cellular and molecular life sciences : CMLS · 2015Review
- Unbalanced expression of CK2 kinase subunits is sufficient to drive epithelial-to-mesenchymal transition by Snail1 induction.Oncogene · 2013Article
- Wnt/beta-catenin signaling and small molecule inhibitors.Current pharmaceutical design · 2013Review
- Asymmetric Localization of CK2α DuringHuman genetics & embryology : current research · 2012Article
- Xenopus paraxial protocadherin inhibits Wnt/β-catenin signalling via casein kinase 2β.EMBO reports · 2012Article
- The dynamic role of beta-catenin in synaptic plasticity.Neuropharmacology · 2012Review
- CK2α is essential for embryonic morphogenesis.Molecular and cellular biochemistry · 2011Article
- Pancreatic adenocarcinoma up-regulated factor (PAUF) enhances the expression of β-catenin, leading to a rapid proliferation of pancreatic cells.Experimental & molecular medicine · 2011Article
- Biochemical and structural characterization of β-catenin interactions with nonphosphorylated and CK2-phosphorylated Lef-1.Journal of molecular biology · 2011Article
- Dynamic expression of a LEF-EGFP Wnt reporter in mouse development and cancer.Genesis (New York, N.Y. : 2000) · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
CK2 is a regulatory kinase implicated in embryonic development and in cancer. Among the CK2 substrates is beta-catenin, a protein with dual function in Wnt signaling and cell adhesion. Previously, we reported that CK2 activity is required for beta-catenin stability and we identified threonine (T) 393 as a major CK2 phosphorylation site in beta-catenin. However, it is not known whether phosphorylation at T393 increases beta-catenin stability and if so, what is the mechanism. In this study we investigate the molecular mechanism of beta-catenin stabilization through phosphorylation at T393. We found that pseudophosphorylation of beta-catenin at T393 resulted in a stable activated form of beta-catenin with decreased affinity for Axin in vitro. This phosphomimetic mutant also displayed decreased regulation by Axin in vivo in a bioassay in Xenopus laevis embryos. In contrast, the binding of T393 pseudophosphorylated beta-catenin to E-cadherin was unaffected. Further analysis showed that pseudophosphorylation at T393 did not prevent beta-catenin phosphorylation by GSK3beta. Interestingly, we found that in the presence of pseudophophorylated beta-catenin and another activated form of beta-catenin, the recruitment of GSK3beta to Axin is enhanced. These findings indicate that phosphorylation of T393 by CK2 may affect the stability of beta-catenin through decreased binding to Axin. In addition, the increased recruitment of GSK3beta to the destruction complex in the presence of activated beta-catenin mutants could be a feedback mechanism to suppress overactive Wnt signaling.
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