ArticleParasitology research2009
Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan.
Article in Parasitology research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed.
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- Opportunistic free-living amoebal pathogens.Pathogens and global health · 2022Article
- Investigating the frequency of free-living amoeba in water resources with emphasis on Acanthamoeba in Bandar Abbas city, Hormozgan province, Iran in 2019-2020.BMC research notes · 2020Article
- An Optimized Most Probable Number (MPN) Method to Assess the Number of Thermophilic Free-Living Amoebae (FLA) in Water Samples.Pathogens (Basel, Switzerland) · 2020Article
- Genotyping by Sequencing of Acanthamoeba and Naegleria Isolates from the Thermal Pool Distributed Throughout Turkey.Acta parasitologica · 2020Article
- Isolation ofPathogens (Basel, Switzerland) · 2020Article
- Occurrence of Infected Free-Living Amoebae in Cooling Towers of Southern Brazil.Current microbiology · 2017Article
- Allovahlkampfia spelaea Causing Keratitis in Humans.PLoS neglected tropical diseases · 2016Article
- Genotypic, physiological, and biochemical characterization of potentially pathogenic Acanthamoeba isolated from the environment in Cairo, Egypt.Parasitology research · 2016Article
- Pharmacy Students' Knowledge Assessment of Naegleria fowleri Infection.Scientifica · 2016Article
- Isolation and identification of pathogenic free-living amoeba from surface and tap water of Shiraz City using morphological and molecular methods.Parasitology research · 2016Article
- Occurrence and molecular characterization of free-living amoeba species (Acanthamoeba, Hartmannella, and Saccamoeba limax) in various surface water resources of Iran.Parasitology research · 2015Article
- Occurrence and Control of Legionella in Recycled Water Systems.Pathogens (Basel, Switzerland) · 2015Review
- Genotypic characterization of amoeba isolated from Acanthamoeba keratitis in Poland.Parasitology research · 2015Article
- Soil is the origin for the presence of Naegleria fowleri in the thermal recreational waters.Parasitology research · 2015Article
- Emerging Threats for Human Health in Poland: Pathogenic Isolates from Drug Resistant Acanthamoeba Keratitis Monitored in terms of Their In Vitro Dynamics and Temperature Adaptability.BioMed research international · 2015Article
- Surveillance of parasitic Legionella in surface waters by using immunomagnetic separation and amoebae enrichment.Pathogens and global health · 2015Article
- Application of TaqMan qPCR for the detection and monitoring of Naegleria species in reservoirs used as a source for drinking water.Parasitology research · 2014Article
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5 authors.
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Abstract
Here, we carried out a survey to determine the prevalence of free-living amoebae (FLA) in tap-water sources from rivers and water treatment plants located in Osaka Prefecture, Japan. A total of 374 raw water samples were collected from 113 sampling points. The samples were filtrated and transferred to non-nutrient agar plates seeded with a heat-killed suspension of Escherichia coli and incubated for 2 to 7 days at 30 degrees C or 42 degrees C. The plates were examined by microscopy to morphologically identify FLA families, and polymerase chain reaction and sequence analysis were then performed to define the species of the detected Naegleria and Acanthamoeba isolates. A total of 257 of 374 samples (68.7%) were positive for FLA by microscopy, and among these there were 800 FLA isolates, including Acanthamoeba and Naegleria species. Sequence analysis identified five Acanthamoeba spp. isolates of the known pathogenic T4 genotype and 43 Naegleria australiensis isolates, a reported pathogen to mice and also of concern as a potential pathogen to humans. Our results suggest a wide distribution of FLA, including potential pathogenic species, in tap-water sources of western Japan.
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