ArticleJournal of molecular and genetic medicine : an international journal of biomedical research2009
Molecular quantitation of H9N2 avian influenza virus in various organs of broiler chickens using TaqMan real time PCR.
Article in Journal of molecular and genetic medicine : an international journal of biomedical research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Rapid Detection Assay for Infectious Bronchitis Virus Using Real-Time Reverse Transcription Recombinase-Aided Amplification.Viruses · 2025Article
- The H9N2 avian influenza virus increases APEC adhesion to oviduct epithelia by viral NS1 protein-mediated activation of the TGF-β pathway.Journal of virology · 2024Article
- Development of a Rapid Fluorescent Diagnostic System for Early Detection of the Highly Pathogenic Avian Influenza H5 Clade 2.3.4.4 Viruses in Chicken Stool.International journal of molecular sciences · 2022Article
- Improved Whole Gamma Irradiated Avian Influenza Subtype H9N2 Virus Vaccine Using Trehalose and Optimization of Vaccination Regime on Broiler Chicken.Frontiers in veterinary science · 2022Article
- Effect of Co-infection of Low Pathogenic Avian Influenza H9N2 Virus and Avian PathogenicFrontiers in veterinary science · 2022Article
- Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate.Veterinary research · 2019Article
- Effect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus HVeterinary research forum : an international quarterly journal · 2019Article
- A G1-lineage H9N2 virus with oviduct tropism causes chronic pathological changes in the infundibulum and a long-lasting drop in egg production.Veterinary research · 2018Article
- A brief summary of the epidemiology and genetic relatedness of avian influenza H9N2 virus in birds and mammals in the Middle East and North Africa.Epidemiology and infection · 2017Review
- Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2).Virusdisease · 2016Article
- Modification of the hemagglutinin cleavage site allows indirect activation of avian influenza virus H9N2 by bacterial staphylokinase.Virology · 2015Article
- A novel activation mechanism of avian influenza virus H9N2 by furin.Journal of virology · 2014Article
- Characterization of recombinant H9N2 influenza viruses isolated from wild ducks in China.Veterinary microbiology · 2013Article
- Matriptase, HAT, and TMPRSS2 activate the hemagglutinin of H9N2 influenza A viruses.Journal of virology · 2013Article
- Systemic distribution of different low pathogenic avian influenza (LPAI) viruses in chicken.Virology journal · 2013Article
- Genetic analysis of polymerase complex (PA, PB1 and PB2) genes of H9N2 avian influenza viruses from Iran (1999 to 2009).Asian Pacific journal of tropical biomedicine · 2012Article
- Systemic virus distribution and host responses in brain and intestine of chickens infected with low pathogenic or high pathogenic avian influenza virus.Virology journal · 2012Article
- Sequence and phylogenetic analysis of the haemagglutinin genes of H9N2 avian influenza viruses isolated from commercial chickens in Iran.Tropical animal health and production · 2010Article
- Monitoring viral RNA in infected cells with LNA flow-FISH.RNA (New York, N.Y.) · 2010Article
- A genetically engineered waterfowl influenza virus with a deletion in the stalk of the neuraminidase has increased virulence for chickens.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the past decade, H9N2 low pathogenic avian influenza virus (LPAI) has caused considerable economic loss due to decreased production, increased mortality and the cost of vaccination in Iranian poultry industry. Because of widespread occurrence of this disease and the virus potential to mutate to highly-pathogenic (HP) form and transmission to humans, it is, therefore, imperative to understand the pathogenesis and properties of these viruses. In this study, a two step TaqMan real time PCR assay was performed for the quantitation of A/chicken/Iran/772/1998(H(9)N(2)) virus in various organs of broiler chickens at different days post inoculation (DPI). Forty 5-week-old commercial broiler chickens were inoculated with the virus. Five chickens were randomly selected on days 1, 3, 6 and 9 PI. Their trachea, lungs, spleen, kidneys, pancreas, blood and faeces were collected for virus detection. A PCR test was performed and the positive samples were used for quantitative real time PCR assay. The result of RT-PCR assay showed the presence of the virus in trachea (40%, 33%), lungs (20%, 66.6%) and spleen (20%, 50%) of infected chickens on days 3 and 6 PI, respectively. The virus was also detected in the kidneys of inoculated chickens on 3 (40%), 6 (60%) and 9 (100%) DPI. In faecal samples the virus was only detected on day 6 PI (83.3%). The molecular quantitation of AIV showed that the AIV titre in the trachea, lungs and spleen of chickens at 3 DPI is lower than the AIV titre at 6 DPI in these organs. The highest titre was observed in the faeces. The AIV titre in all organs of the birds which died at 6 DPI was higher than those of the same organs in the other experimental birds.
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