ArticleInfection and immunity2009
Roles of RseB, sigmaE, and DegP in virulence and phase variation of colony morphotype of Vibrio vulnificus.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus.Nucleic acids research · 2025Article
- Effect of capsular polysaccharide phase variation on biofilm formation, motility and gene expression in Vibrio vulnificus.Gut pathogens · 2024Article
- Polymorphism and mutational diversity of virulence (vcgCPI/vcgCPE) and resistance determinants (aac(3)-IIa, (aacC2, strA, Sul 1, and 11) among human pathogenic Vibrio species recovered from surface waters in South-Western districts of Uganda.Journal, genetic engineering & biotechnology · 2023Article
- Tools to Enumerate and Predict Distribution Patterns of EnvironmentalMicroorganisms · 2023Review
- Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus.NPJ biofilms and microbiomes · 2021Article
- Structure, Function, and Regulation of the Essential Virulence Factor Capsular Polysaccharide ofInternational journal of molecular sciences · 2020Review
- Phylogeny ofFrontiers in microbiology · 2017Article
- Post-Genomic Analysis of Members of the Family Vibrionaceae.Microbiology spectrum · 2015Review
- Transcriptional profiles of Burkholderia pseudomallei reveal the direct and indirect roles of Sigma E under oxidative stress conditions.BMC genomics · 2014Article
- Alternative sigma factor RpoE is important for Vibrio parahaemolyticus cell envelope stress response and intestinal colonization.Infection and immunity · 2014Article
- Genetic analysis and prevalence studies of the brp exopolysaccharide locus of Vibrio vulnificus.PloS one · 2014Article
- Role of Bordetella pertussis RseA in the cell envelope stress response and adenylate cyclase toxin release.Pathogens and disease · 2013Article
- Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi.PloS one · 2012Article
- Recognition of β-strand motifs by RseB is required for σ(E) activity in Escherichia coli.Journal of bacteriology · 2011Article
- Naturally occurring single amino acid replacements in a regulatory protein alter streptococcal gene expression and virulence in mice.The Journal of clinical investigation · 2011Article
- USER friendly cloning coupled with chitin-based natural transformation enables rapid mutagenesis of Vibrio vulnificus.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Vibrio vulnificus is an estuarine bacterium capable of causing serious and often fatal wound infections and primary septicemia. We used alkaline phosphatase insertion mutagenesis to identify genes necessary for the virulence of this pathogen. One mutant had an in-frame fusion of 'phoA to the gene encoding RseB, a periplasmic negative regulator of the alternative sigma factor sigma(E). sigma(E) controls an extensive regulon involved in responding to cell envelope stresses. Colonies of the rseB mutant were less opaque than wild-type colonies and underwent phase variation between translucent and opaque morphologies. rseB mutants were attenuated for virulence in subcutaneously inoculated iron-dextran-treated mice. To obtain insight into the role of rseB and the extracytoplasmic stress response in V. vulnificus, mutants with defined mutations in rseB and two important members of the extracytoplasmic stress regulon, rpoE and degP, were constructed for analysis of virulence, colony morphology, and stress-associated phenotypes. Deletion of rseB caused reversible phase variation in the colony morphotype that was associated with extracellular polysaccharides. Translucent and transparent morphotype strains were attenuated for virulence. rpoE and degP deletion mutants were sensitive to membrane-perturbing agents and heat but were not significantly attenuated for V. vulnificus virulence in mice. These results reveal complex relationships between regulation of the extracytoplasmic stress response, exopolysaccharides, and the virulence of V. vulnificus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.