ReviewMolecular biotechnology2009
Nucleic acids electrotransfer-based gene therapy (electrogenetherapy): past, current, and future.
Review in Molecular biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed.
- Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Importance of the electrophoresis and pulse energy for siRNA-mediated gene silencing by electroporation in differentiated primary human myotubes.Biomedical engineering online · 2024Article
- Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses.Frontiers in bioengineering and biotechnology · 2024Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Gene Therapy of Chronic Limb-Threatening Ischemia: Vascular Medical Perspectives.Journal of clinical medicine · 2022Review
- OpenEP: an open-source simulator for electroporation-based tumor treatments.Scientific reports · 2021Article
- Possible molecular and cellular mechanisms at the basis of atmospheric electromagnetic field bioeffects.International journal of biometeorology · 2021Review
- Current Progress in Electrotransfection as a Nonviral Method for Gene Delivery.Molecular pharmaceutics · 2018Article
- Conductivity Rise During Irreversible Electroporation: True Permeabilization or Heat?Cardiovascular and interventional radiology · 2018Article
- Electropermeabilization of Inner and Outer Cell Membranes with Microsecond Pulsed Electric Fields: Quantitative Study with Calcium Ions.Scientific reports · 2017Article
- Shock Wave-Induced Damage and Poration in Eukaryotic Cell Membranes.The Journal of membrane biology · 2017Article
- Demonstration of the Protein Involvement in Cell Electropermeabilization using Confocal Raman Microspectroscopy.Scientific reports · 2017Article
- Direct Current Helium Plasma forPlasma medicine · 2017Article
- Dielectrophoresis study of temporal change in internal conductivity of single CHO cells after electroporation by pulsed electric fields.Biomicrofluidics · 2017Article
- The promising alliance of anti-cancer electrochemotherapy with immunotherapy.Cancer metastasis reviews · 2016Review
- Mapping of bionic array electric field focusing in plasmid DNA-based gene electrotransfer.Gene therapy · 2016Article
- Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses.Scientific reports · 2016Article
- Comparison of Different Electroporation Parameters on Transfection Efficiency of Sheep Testicular Cells.Cell journal · 2016Article
- Manipulation of cell membrane using carbon nanotube scaffold as a photoresponsive stimuli generator.Science and technology of advanced materials · 2014Article
- Electroporation formulation for cell therapy.Methods in molecular biology (Clifton, N.J.) · 2014Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
About 25 years after the publication of the first report on gene transfer in vitro in cultured cells by the means of electric pulses delivery, reversible cell electroporation for gene transfer and gene therapy (DNA electrotransfer) is at a cross in its development. Present knowledge on the effects of cell exposure to appropriate electric field pulses, particularly at the level of the cell membrane, is reported here. The importance of the models of electric field distribution in tissues and of the correct choice of electrodes and applied voltages is highlighted. The mechanisms involved in DNA electrotransfer, which include cell electropermeabilization and DNA electrophoresis, are also surveyed. This knowledge has allowed developing new nucleic acids electrotransfer conditions using combinations of permeabilizing pulses of high voltage and short duration, and of electrophoretic pulses of low voltage and long duration, which are very efficient and safer. Feasibility of electric pulses delivery for gene transfer in humans is discussed taking into account that electric pulses delivery is already regularly used for localized drug delivery in the treatment of cutaneous and subcutaneous solid tumors by electrochemotherapy. Because recent technological developments made DNA electrotransfer more and more efficient and safer, this non-viral gene therapy approach is now ready to reach the clinical stage. A good understanding of DNA electrotransfer principles and the respect of safe procedures will be key elements for a successful future transfer DNA electrotransfer into the clinics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.