Evidence map›Paper›PMID 19562512›Full record

ArticleMolecular biology reports2010

Isolation and heterologous transformation analysis of a pollen-specific promoter from wheat (Triticum aestivum L.).

Ling Chen, Zhiming Tu, Javeed Hussain, Ling Cong, Yinjun Yan, Lian Jin, Guangxiao Yang, Guangyuan He

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Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

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0cells of the map it votes in
15citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Ling ChenChina-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, The Genetic Engineering International Cooperation Base of Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Ministry of Education, Huazhong University of Science and Technology (HUST), Luoyo Road 1037, 430074, Wuhan, China.
Zhiming Tu
Javeed Hussain
Ling Cong
Yinjun Yan
Lian Jin
Guangxiao Yang
Guangyuan He

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The promoter of a pollen-specific gene TaPSG719 was isolated from wheat (Triticum aestivum L.) by inverse-PCR (IPCR). Sequence analysis revealed that the promoter contains two cis-acting elements (AGAAA and GTGA) known to confer anther/pollen-specific gene expression which suggests that the promoter of TaPSG719 gene is a pollen-specific one. To ascertain the regulatory function of TaPSG719 promoter, two deleted fragments (-1,776 to -1 bp and -1,019 to -1 bp) were fused to the beta-glucuronidase (GUS) gene and transformed into tobacco plants. Similar GUS expression patterns were observed in all transformed plants and its activity was detected exclusively in pollen. No GUS activity in any other floral or vegetative tissue was observed. The results confirm that TaPSG719 promoter is pollen-specific and active during the middle stages of pollen development till anther matured, and it can drive pollen-specific gene expression across the species.

Indexed as

Promoter Regions, GeneticTransformation, GeneticBase SequenceCloning, MolecularDNA, PlantGene Expression Regulation, PlantGlucuronidaseMolecular Sequence DataNicotianaOrgan SpecificityPlants, Genetically ModifiedPollenSequence Analysis, DNATriticumDNA, PlantGlucuronidase

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.