ArticleTransgenic research2010
Plastid transformation in eggplant (Solanum melongena L.).
Article in Transgenic research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 78 citations in OpenAlex.
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Map-based cloning of LPD, a major gene positively regulates leaf prickle development in eggplant.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Article
- Insights into plastome ofSaudi journal of biological sciences · 2022Article
- Gene introduction approaches in chloroplast transformation and its applications.Journal, genetic engineering & biotechnology · 2021Review
- Plant Platforms for Efficient Heterologous Protein Production.Biotechnology and bioprocess engineering : BBE · 2021Review
- Plastome engineering in vegetable crops: current status and future prospects.Molecular biology reports · 2020Review
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- A novel strategy for promoting homoplasmic plastid transformant production using the barnase-barstar system.Plant biotechnology (Tokyo, Japan) · 2020Article
- A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant.BMC plant biology · 2020Article
- Production of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development.Plant biotechnology journal · 2019Article
- Stable plastid transformation inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Chloroplast genomes: diversity, evolution, and applications in genetic engineering.Genome biology · 2016Review
- Plastid genomics in horticultural species: importance and applications for plant population genetics, evolution, and biotechnology.Frontiers in plant science · 2015Review
- Article
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Plastid genetic engineering in Solanaceae.Protoplasma · 2012Review
- Stable plastid transformation for high-level recombinant protein expression: promises and challenges.Journal of biomedicine & biotechnology · 2012Article
- Plastid biotechnology for crop production: present status and future perspectives.Plant molecular biology · 2011Article
- Spontaneous spectinomycin resistance mutations detected after biolistic transformation of Daucus carota L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a method for plastid transformation in eggplant (Solanum melongena L.), a solanaceous plant species. Plastid transformation in eggplant was achieved by bombardment of green stem segments with pPRV111A plastid expression vector carrying the aadA gene encoding aminoglycoside 3''-adenylyltransferase. Biolistic delivery of the pPRV111A plasmid yielded transplastomic plants at a frequency of two per 21 bombarded plates containing 25 stem explants each. Integration of the aadA gene in the plastome was verified by PCR analysis and also by Southern blotting using 16S rDNA (targeting sequence) and the aadA gene as a probe. Transplastomic expression of the aadA gene was verified by RT-PCR. The development of transplastomic technology in eggplant may open up exciting possibilities for novel gene introduction and expression in the engineered plastome for agronomic or pharmaceutical traits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.