ArticleParasitology research2009
In vitro antifilarial activity of glutathione S-transferase inhibitors.
Article in Parasitology research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
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- Anti-giardial therapeutic potential of dichloromethane extracts of Zingiber officinale and Curcuma longa in vitro and in vivo.Parasitology research · 2016Article
- Molecular epidemiology, phylogeny and evolution of the filarial nematode Wuchereria bancrofti.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2014Review
- Plumbagin modulates leukemia cell redox status.Molecules (Basel, Switzerland) · 2014Article
- Identification of plumbagin and sanguinarine as effective chemotherapeutic agents for treatment of schistosomiasis.International journal for parasitology. Drugs and drug resistance · 2013Article
- Current drug targets for helminthic diseases.Parasitology research · 2013Review
- Molecular evidence of curcumin-induced apoptosis in the filarial worm Setaria cervi.Parasitology research · 2012Article
- Virtual screening and in vitro assay of potential drug like inhibitors from spices against glutathione-S-transferase of filarial nematodes.Journal of molecular modeling · 2012Article
- Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin.Plant cell reports · 2012Article
- Phylogenetic study of Setaria cervi based on mitochondrial cox1 gene sequences.Parasitology research · 2012Article
- Studies on filarial GST as a target for antifilarial drug development-in silico and in vitro inhibition of filarial GST by substituted 1,4-naphthoquinones.Journal of molecular modeling · 2011Article
- Biochemical studies on glutathione S-transferase from the bovine filarial worm Setaria digitata.Parasitology research · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Female adult bovine filarial worms Setaria digitata were extracted with phosphate-buffered saline (pH 7.4) and glutathione S-transferase (GST) activity and protein content were determined. The protein content, GST enzyme activity, and specific activity were 10.61 +/- 3.41 mg ml(-1), 0.09 +/- 0.019 micromol min(-1) ml(-1), and 0.009 +/- 0.002 micromol min(-1) mg(-1) protein, respectively. The GST inhibition studies were performed with and without the inhibitors resulted from earlier molecular docking studies viz., ethacrynic acid, plumbagin, and curcumin for which the IC(50) values were 19.42, 51.41, and 114.86 microM, respectively. The in vitro macrofilaricidal activity of these molecules was studied by worm motility and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay at 24- and 48-h incubation. Plumbagin and ethacrynic acid showed 100% inhibition in worm motility at lower concentrations of 3.19 and 6.6 microM, respectively, at 48-h incubation while curcumin was effective at 54.29 microM. In MTT reduction assay, the ED(50) values (50% inhibition in formazan formation) for plumbagin, ethacrynic acid, and curcumin at 48-h incubation were 1.20, 2.48, and 19.86 microM, respectively. MTT reduction assay showed that plumbagin was the most effective in killing the adult S. digitata worms followed by ethacrynic acid and curcumin. In conclusion, all the three molecules selected by molecular modeling and docking studies inhibited the GST enzyme isolated from S. digitata and exhibited macrofilaricidal activity in vitro.
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