ArticleFrontiers in neuroengineering2009
Design and adsorption of modular engineered proteins to prepare customized, neuron-compatible coatings.
Article in Frontiers in neuroengineering, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Silk-Elastin-like Polymers for Acute Intraparenchymal Treatment of the Traumatically Injured Spinal Cord: A First Systematic Experimental Approach.Pharmaceutics · 2022Article
- Hyaluronan and elastin-like protein (HELP) gels significantly improve microsphere retention in the myocardium.Biomaterials science · 2022Article
- Investigating the interplay between substrate stiffness and ligand chemistry in directing mesenchymal stem cell differentiation within 3D macro-porous substrates.Biomaterials · 2018Article
- Engineering Hydrogel Microenvironments to Recapitulate the Stem Cell Niche.Annual review of biomedical engineering · 2018Review
- Creating cellular patterns using genetically engineered, gold- and cell-binding polypeptides.Biointerphases · 2016Article
- Use of protein-engineered fabrics to identify design rules for integrin ligand clustering in biomaterials.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Protein-engineered scaffolds for in vitro 3D culture of primary adult intestinal organoids.Biomaterials science · 2015Article
- Aligned-Braided Nanofibrillar Scaffold with Endothelial Cells Enhances Arteriogenesis.ACS nano · 2015Article
- Microfluidic gradients reveal enhanced neurite outgrowth but impaired guidance within 3D matrices with high integrin ligand densities.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Design of three-dimensional engineered protein hydrogels for tailored control of neurite growth.Acta biomaterialia · 2013Article
- Sequence-specific crosslinking of electrospun, elastin-like protein preserves bioactivity and native-like mechanics.Advanced healthcare materials · 2013Article
- In vivo effects of L1 coating on inflammation and neuronal health at the electrode-tissue interface in rat spinal cord and dorsal root ganglion.Acta biomaterialia · 2012Article
- Functional enhancement of neuronal cell behaviors and differentiation by elastin-mimetic recombinant protein presenting Arg-Gly-Asp peptides.BMC biotechnology · 2012Article
- Cell behavior on a CCN1 functionalized elastin-mimetic protein polymer.Biomaterials · 2012Article
- Protein-engineered biomaterials: nanoscale mimics of the extracellular matrix.Biochimica et biophysica acta · 2011Review
- Neuroengineering tools/applications for bidirectional interfaces, brain-computer interfaces, and neuroprosthetic implants - a review of recent progress.Frontiers in neuroengineering · 2010Article
- Two-component protein-engineered physical hydrogels for cell encapsulation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural prosthetic implants are currently being developed for the treatment and study of both peripheral and central nervous system disorders. Effective integration of these devices upon implantation is a critical hurdle to achieving function. As a result, much attention has been directed towards the development of biocompatible coatings that prolong their in vivo lifespan. In this work, we present a novel approach to fabricate such coatings, which specifically involves the use of surface-adsorbed, nanoscale-designed protein polymers to prepare reproducible, customized surfaces. A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. Physical adsorption isotherms were experimentally determined for these engineered proteins, allowing for direct calculation of the available ligand density present on coated surfaces. As confirmation that ligand density in these engineered systems impacts neuronal cell behavior, we demonstrate that increasing the density of fibronectin-derived RGD ligands on coated surfaces while maintaining uniform protein surface coverage results in enhanced neurite extension of PC-12 cells. Therefore, this engineered protein adsorption approach allows for the facile preparation of tunable, quantifiable, and reproducible surfaces for in vitro studies of cell-ligand interactions and for potential application as coatings on neural implants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.