ArticleThe Journal of biological chemistry2009
The interaction of mitochondrial iron with manganese superoxide dismutase.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 64 citations in OpenAlex.
- Effects of a second iron injection and sire line on growth performance, hemoglobin levels, antioxidative status, and whole-body iron retention in piglets.Journal of animal science · 2026Article
- Converging Roles of the Metal Transporter SMF11 and the Ferric Reductase FRE1 in Iron Homeostasis of Candida albicans.Molecular microbiology · 2024Article
- Nutritional Immunity and Fungal Pathogens: a New Role for Manganese.Current clinical microbiology reports · 2024Article
- Glutathione peroxidase 4 suppresses manganese-dependent oxidative stress to reduce colorectal tumorigenesis.Research square · 2024Article
- Hem25p is required for mitochondrial IPP transport in fungi.Nature cell biology · 2023Article
- MTM1 displays a new function in the regulation of nickel resistance in Saccharomyces cerevisiae.Metallomics : integrated biometal science · 2022Article
- Computationally Guided Redesign of a Heme-free Cytochrome with Native-like Structure and Stability.Biochemistry · 2022Article
- The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence.Antioxidants (Basel, Switzerland) · 2022Review
- Molecular Characterization and the Essential Biological Function of the Metal Chaperone Protein MtmA in Aspergillus fumigatus.Applied and environmental microbiology · 2022Article
- Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 inPlants (Basel, Switzerland) · 2022Article
- Prime Real Estate: Metals, Cofactors and MICOS.Frontiers in cell and developmental biology · 2022Review
- Review
- Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation inFrontiers in plant science · 2021Article
- Iron-mediated degradation of ribosomes under oxidative stress is attenuated by manganese.The Journal of biological chemistry · 2020Article
- Review
- Disruption in iron homeostasis and impaired activity of iron-sulfur cluster containing proteins in the yeast model of Shwachman-Diamond syndrome.Cell & bioscience · 2020Article
- A comprehensive mechanistic model of iron metabolism in Saccharomyces cerevisiae.Metallomics : integrated biometal science · 2019Article
- Lysine 68 acetylation directs MnSOD as a tetrameric detoxification complex versus a monomeric tumor promoter.Nature communications · 2019Article
- The mitochondrial ABC transporter Atm1 plays a role in iron metabolism and virulence in the human fungal pathogen Cryptococcus neoformans.Medical mycology · 2018Article
- A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Superoxide dismutase 2 (SOD2) is one of the rare mitochondrial enzymes evolved to use manganese as a cofactor over the more abundant element iron. Although mitochondrial iron does not normally bind SOD2, iron will misincorporate into Saccharomyces cerevisiae Sod2p when cells are starved for manganese or when mitochondrial iron homeostasis is disrupted by mutations in yeast grx5, ssq1, and mtm1. We report here that such changes in mitochondrial manganese and iron similarly affect cofactor selection in a heterologously expressed Escherichia coli Mn-SOD, but not a highly homologous Fe-SOD. By x-ray absorption near edge structure and extended x-ray absorption fine structure analyses of isolated mitochondria, we find that misincorporation of iron into yeast Sod2p does not correlate with significant changes in the average oxidation state or coordination chemistry of bulk mitochondrial iron. Instead, small changes in mitochondrial iron are likely to promote iron-SOD2 interactions. Iron binds Sod2p in yeast mutants blocking late stages of iron-sulfur cluster biogenesis (grx5, ssq1, and atm1), but not in mutants defective in the upstream Isu proteins that serve as scaffolds for iron-sulfur biosynthesis. In fact, we observed a requirement for the Isu proteins in iron inactivation of yeast Sod2p. Sod2p activity was restored in mtm1 and grx5 mutants by depleting cells of Isu proteins or using a dominant negative Isu1p predicted to stabilize iron binding to Isu1p. In all cases where disruptions in iron homeostasis inactivated Sod2p, we observed an increase in mitochondrial Isu proteins. These studies indicate that the Isu proteins and the iron-sulfur pathway can donate iron to Sod2p.
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Registered trials
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