ArticleNucleic acids research2009
Discovering ligands for a microRNA precursor with peptoid microarrays.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 76 citations in OpenAlex.
- Noncanonical Folding of Peptoid Oligomers: Formation of a Closed Conformation in Nonpolar Solvent.Organic letters · 2026Article
- Drug-Like Small Molecules That Inhibit Expression of the Oncogenic MicroRNA-21.ACS chemical biology · 2023Article
- Rational Approach to Identify RNA Targets of Natural Products Enables Identification of Nocathiacin as an Inhibitor of an Oncogenic RNA.ACS chemical biology · 2022Article
- DNA-encoded library versus RNA-encoded library selection enables design of an oncogenic noncoding RNA inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Interbase-FRET binding assay for pre-microRNAs.Scientific reports · 2021Article
- Recognizing and stabilizing miR-21 by chiral ruthenium(II) complexes.BMC chemistry · 2020Article
- miRNA inhibition by proximity-enabled Dicer inactivation.Methods (San Diego, Calif.) · 2019Article
- Decrypting noncoding RNA interactions, structures, and functional networks.Genome research · 2019Review
- Tetracyclines as Inhibitors of Pre-microRNA Maturation: A Disconnection between RNA Binding and Inhibition.ACS medicinal chemistry letters · 2019Article
- Targeting RNA in mammalian systems with small molecules.Wiley interdisciplinary reviews. RNA · 2018Review
- Development and Implementation of an HTS-Compatible Assay for the Discovery of Selective Small-Molecule Ligands for Pre-microRNAs.SLAS discovery : advancing life sciences R & D · 2018Article
- Amikacin Inhibits miR-497 Maturation and Exerts Post-ischemic Neuroprotection.Molecular neurobiology · 2017Article
- A Macrocyclic Peptide Ligand Binds the Oncogenic MicroRNA-21 Precursor and Suppresses Dicer Processing.ACS chemical biology · 2017Article
- Discovery of Inhibitors of MicroRNA-21 Processing Using Small Molecule Microarrays.ACS chemical biology · 2017Article
- Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Microarray-based technologies for the discovery of selective, RNA-binding molecules.Methods (San Diego, Calif.) · 2016Review
- Identification of Spermidine Binding Site in T-box Riboswitch Antiterminator RNA.Chemical biology & drug design · 2016Article
- Small molecules targeting microRNA for cancer therapy: Promises and obstacles.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Factors that influence T box riboswitch efficacy and tRNA affinity.Bioorganic & medicinal chemistry · 2015Article
- Tethering in RNA: an RNA-binding fragment discovery tool.Molecules (Basel, Switzerland) · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have screened peptoid microarrays to identify specific ligands for the RNA hairpin precursor of miR-21, a microRNA involved in cancer and heart disease. Microarrays were printed by spotting a library of 7680 N-substituted oligoglycines (peptoids) onto glass slides. Two compounds on the array specifically bind RNA having the sequence and predicted secondary structure of the miR-21 precursor hairpin and have specific affinity for the target in solution. Their binding induces a conformational change around the hairpin loop, and the most specific compound recognizes the loop sequence and a bulged uridine in the proximal duplex. Functional groups contributing affinity and specificity were identified, and by varying a critical methylpyridine group, a compound with a dissociation constant of 1.9 microM for the miR-21 precursor hairpin and a 20-fold discrimination against a closely-related hairpin was created. This work describes a systematic approach to discovery of ligands for specific pre-defined novel RNA structures. It demonstrates discovery of new ligands for an RNA for which no specific lead compounds were previously known by screening a microarray of small molecules.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.