ArticleMolecular cell2009
Mechanical constraints on Hin subunit rotation imposed by the Fis/enhancer system and DNA supercoiling during site-specific recombination.
Article in Molecular cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Negative DNA supercoiling enhances DARS2 binding of DNA-bending protein IHF in the activation of Fis-dependent ATP-DnaA production.Nucleic acids research · 2025Article
- Direct observation of subunit rotation during DNA strand exchange by serine recombinases.Nature communications · 2024Article
- Regulation of pneumococcal epigenetic and colony phases by multiple two-component regulatory systems.PLoS pathogens · 2020Article
- Snapshots of a molecular swivel in action.Nucleic acids research · 2018Article
- Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination.Micromachines · 2018Review
- The regulatory role of DNA supercoiling in nucleoprotein complex assembly and genetic activity.Biophysical reviews · 2016Review
- Molecular Mechanism of Facilitated Dissociation of Fis Protein from DNA.Journal of the American Chemical Society · 2016Article
- Controlled rotation mechanism of DNA strand exchange by the Hin serine recombinase.Scientific reports · 2016Article
- Controlling tetramer formation, subunit rotation and DNA ligation during Hin-catalyzed DNA inversion.Nucleic acids research · 2015Article
- Site-specific DNA Inversion by Serine Recombinases.Microbiology spectrum · 2015Article
- A proposed mechanism for IS607-family serine transposases.Mobile DNA · 2013Article
- Multiple interfaces between a serine recombinase and an enhancer control site-specific DNA inversion.eLife · 2013Article
- Crystal structure of an intermediate of rotating dimers within the synaptic tetramer of the G-segment invertase.Nucleic acids research · 2013Article
- The site-specific integration reaction of Listeria phage A118 integrase, a serine recombinase.Mobile DNA · 2013Article
- Gated rotation mechanism of site-specific recombination by ϕC31 integrase.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Effect of iacP mutation on flagellar phase variation in Salmonella enterica serovar typhimurium strain UK-1.Journal of bacteriology · 2012Article
- Intrasubunit and intersubunit interactions controlling assembly of active synaptic complexes during Hin-catalyzed DNA recombination.Journal of molecular biology · 2011Article
- Structural basis for catalytic activation of a serine recombinase.Structure (London, England : 1993) · 2011Article
- Topoisomerases and site-specific recombinases: similarities in structure and mechanism.Critical reviews in biochemistry and molecular biology · 2010Review
- Sin resolvase catalytic activity and oligomerization state are tightly coupled.Journal of molecular biology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Hin, a member of the serine family of site-specific recombinases, regulates gene expression by inverting a DNA segment. DNA inversion requires assembly of an invertasome complex in which a recombinational enhancer DNA segment bound by the Fis protein associates with the Hin synaptic complex at the base of a supercoiled DNA branch. Each of the four Hin subunits becomes covalently joined to the cleaved DNA ends, and DNA exchange occurs by translocation of a Hin subunit pair within the tetramer. We show here that, although the Hin tetramer forms a bidirectional molecular swivel, the Fis/enhancer system determines both the direction and number of subunit rotations. The chirality of supercoiling directs rotational direction, and the short DNA loop stabilized by Fis-Hin contacts limit rotational processivity, thereby ensuring that the DNA strands religate in the recombinant configuration. We identify multiple rotational conformers that are formed under different supercoiling and solution conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.