ArticleGenome biology2009
Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea.
Article in Genome biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Snapshots of Motion: A Novel Structural Intermediate Reveals Conserved Dynamics in Archaeal DNA Ligases.Proteins · 2026Article
- Radiation-Resistant Microbes: Pioneers for Life Beyond Earth.Current microbiology · 2026Review
- The Thermococcales as a model system: historical perspectives and emerging tools.Journal of bacteriology · 2025Review
- Tissue-Cultured Chondrocytes Survive After Irradiation in 1300 Gy Dose.Biomedicines · 2025Article
- Microbiome analysis of the restricted bacteria in radioactive element-containing water at the Fukushima Daiichi Nuclear Power Station.Applied and environmental microbiology · 2024Article
- The impact ofJournal of genomics · 2024Article
- NADPApplied and environmental microbiology · 2023Article
- A moderately thermophilic origin of a novel family of marine group II euryarchaeota from deep ocean.iScience · 2023Article
- Taxonomical composition and functional analysis of biofilms sampled from a nuclear storage pool.Frontiers in microbiology · 2023Article
- Single-Molecule/Cell Analyses Reveal Principles of Genome-Folding Mechanisms in the Three Domains of Life.International journal of molecular sciences · 2021Review
- In silico identification of novel open reading frames in Plasmodium falciparum oocyte and salivary gland sporozoites using proteogenomics framework.Malaria journal · 2021Article
- Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool.Microorganisms · 2020Article
- The global distribution and evolutionary history of the pT26-2 archaeal plasmid family.Environmental microbiology · 2019Article
- Diversity and Niche of Archaea in Bioremediation.Archaea (Vancouver, B.C.) · 2018Review
- Extremophilic Microfactories: Applications in Metal and Radionuclide Bioremediation.Frontiers in microbiology · 2018Review
- Microbial radiation-resistance mechanisms.Journal of microbiology (Seoul, Korea) · 2017Review
- Radiation resistance in thermophiles: mechanisms and applications.World journal of microbiology & biotechnology · 2017Review
- Comparative Genomics ofFrontiers in microbiology · 2017Article
- Archaeal RNA polymerase arrests transcription at DNA lesions.Transcription · 2017Article
- Host Plant Compatibility Shapes the Proteogenome ofFrontiers in microbiology · 2017Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThermococcus gammatolerans was isolated from samples collected from hydrothermal chimneys. It is one of the most radioresistant organisms known amongst the Archaea. We report the determination and annotation of its complete genome sequence, its comparison with other Thermococcales genomes, and a proteomic analysis.
resultsT. gammatolerans has a circular chromosome of 2.045 Mbp without any extra-chromosomal elements, coding for 2,157 proteins. A thorough comparative genomics analysis revealed important but unsuspected genome plasticity differences between sequenced Thermococcus and Pyrococcus species that could not be attributed to the presence of specific mobile elements. Two virus-related regions, tgv1 and tgv2, are the only mobile elements identified in this genome. A proteogenome analysis was performed by a shotgun liquid chromatography-tandem mass spectrometry approach, allowing the identification of 10,931 unique peptides corresponding to 951 proteins. This information concurrently validates the accuracy of the genome annotation. Semi-quantification of proteins by spectral count was done on exponential- and stationary-phase cells. Insights into general catabolism, hydrogenase complexes, detoxification systems, and the DNA repair toolbox of this archaeon are revealed through this genome and proteome analysis.
conclusionsThis work is the first archaeal proteome investigation done at the stage of primary genome annotation. This archaeon is shown to use a large variety of metabolic pathways even under a rich medium growth condition. This proteogenomic study also indicates that the high radiotolerance of T. gammatolerans is probably due to proteins that remain to be characterized rather than a larger arsenal of known DNA repair enzymes.
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