ArticleVirology journal2009
Respiratory viral infections detected by multiplex PCR among pediatric patients with lower respiratory tract infections seen at an urban hospital in Delhi from 2005 to 2007.
Article in Virology journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 1 of them a synthesis that pooled it.
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Who cites it
70 citing papers in PubMed, 1 synthesis or guideline pooled it, 145 citations in OpenAlex.
- Viral etiology of hospitalized acute lower respiratory infections in children under 5 years of age -- a systematic review and meta-analysis.Croatian medical journal · 2013Pooled it
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- Human Metapneumovirus (HMPV): Advances in Diagnosis, Molecular Epidemiology, and Clinical Impact of an Underrecognized Respiratory Virus.Diagnostics (Basel, Switzerland) · 2026Review
- The prevalence and role of human respiratory syncytial virus in pediatric respiratory tract infections: a systematic review and meta-analysis of global data.EClinicalMedicine · 2026Article
- Clinical and Etiological Spectrum of Viral Pneumonia in Critically Ill Children: A Retrospective Study.Indian pediatrics · 2026Article
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- Incidence of respiratory viral infections in adults following hematopoietic stem cell transplant (HSCT) at a tertiary care hospital in India.Virusdisease · 2025Article
- Respiratory virus infection dynamics and genomic surveillance to detect seasonal influenza subtypes in wastewater: A longitudinal study in Bengaluru, India.PLOS global public health · 2025Article
- Molecular epidemiology of influenza, respiratory syncytial virus, SARS-CoV-2, other respiratory viruses and bacteria among children 0-2-year-olds in West Bengal: a one-year influenza-like illness surveillance study (2022-2023).Frontiers in epidemiology · 2025Article
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- Prevalence and Surveillance of Influenza Viruses in Hospitalized Patients with Respiratory Infections in and Around Chennai, Southern Part of India.Indian journal of microbiology · 2024Article
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- Acute Viral Bronchiolitis: A Narrative Review.Journal of pediatric intensive care · 2023Review
- Characterization of T Helper 1 and 2 Cytokine Profiles in Newborns of Mothers with COVID-19.Biomedicines · 2023Article
- Reclassification of respiratory syncytial virus genotypes in India.Virusdisease · 2023Article
- Performance Evaluation of TaqMan SARS-CoV-2, Flu A/B, RSV RT-PCR Multiplex Assay for the Detection of Respiratory Viruses.Infection and drug resistance · 2022Article
- Evaluation of viral respiratory pathogens in children aged under five hospitalized with lower respiratory tract infections.Northern clinics of Istanbul · 2022Article
- Molecular epidemiology of respiratory syncytial virus among children and adults in India 2016 to 2018.Virus genes · 2021Article
- Clinico-virological Profile, Intensive Care Needs, and Outcome of Infants with Acute Viral Bronchiolitis: A Prospective Observational Study.Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine · 2021Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundAcute lower respiratory tract infections (ALRI) are the major cause of morbidity and mortality in young children worldwide. Information on viral etiology in ALRI from India is limited. The aim of the present study was to develop a simple, sensitive, specific and cost effective multiplex PCR (mPCR) assay without post PCR hybridization or nested PCR steps for the detection of respiratory syncytial virus (RSV), influenza viruses, parainfluenza viruses (PIV1-3) and human metapneumovirus (hMPV). Nasopharyngeal aspirates (NPAs) were collected from children with ALRI < or = 5 years of age. The sensitivity and specificity of mPCR was compared to virus isolation by centrifugation enhanced culture (CEC) followed by indirect immunofluorescence (IIF).
resultsFrom April 2005-March 2007, 301 NPAs were collected from children attending the outpatient department or admitted to the ward of All India Institute of Medical Sciences hospital at New Delhi, India. Multiplex PCR detected respiratory viruses in 106 (35.2%) of 301 samples with 130 viruses of which RSV was detected in 61, PIV3 in 22, PIV2 in 17, hMPV in 11, PIV1 in 10 and influenza A in 9 children. CEC-IIF detected 79 viruses only. The sensitivity of mPCR was 0.1TCID50 for RSV and influenza A and 1TCID50 for hMPV, PIV1, PIV2, PIV3 and Influenza B. Mixed infections were detected in 18.8% of the children with viral infections, none detected by CEC-IIF. Bronchiolitis was significantly associated with both total viral infections and RSV infection (p < 0.05). History of ARI in family predisposed children to acquire viral infection (p > 0.05).
conclusionMultiplex PCR offers a rapid, sensitive and reasonably priced diagnostic method for common respiratory viruses.
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