ArticleBMC molecular biology2009
Selection and evaluation of reference genes for improved interrogation of microbial transcriptomes: case study with the extremophile Acidithiobacillus ferrooxidans.
Article in BMC molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Biofilm engineering through c-di-GMP tuning boosts bioleaching efficiency and arsenic tolerance inApplied and environmental microbiology · 2026Article
- Microbial succession and assembly shaped by sulfur, spatial partitioning, and water flow in a volcanic acidic river of northern Patagonia.The ISME journal · 2026Article
- Nutrient structure dynamics and microbial communities at the water-sediment interface in an extremely acidic lake in northern Patagonia.Frontiers in microbiology · 2024Article
- Influence of mobile genetic elements and insertion sequences in long- and short-term adaptive processes of Acidithiobacillus ferrooxidans strains.Scientific reports · 2023Article
- Validation of housekeeping genes as internal controls for gene expression studies on biofilm formation in Bacillus velezensis.Applied microbiology and biotechnology · 2022Article
- Genomic evolution of the class Acidithiobacillia: deep-branching Proteobacteria living in extreme acidic conditions.The ISME journal · 2021Article
- Evolution of Type IV CRISPR-Cas Systems: Insights from CRISPR Loci in Integrative Conjugative Elements ofThe CRISPR journal · 2021Article
- The substrate-dependent regulatory effects of the AfeI/R system in Acidithiobacillus ferrooxidans reveals the novel regulation strategy of quorum sensing in acidophiles.Environmental microbiology · 2021Article
- Article
- Genetically similar temperate phages form coalitions with their shared host that lead to niche-specific fitness effects.The ISME journal · 2020Article
- Identification and Unusual Properties of the Master Regulator FNR in the Extreme AcidophileFrontiers in microbiology · 2019Article
- Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors.Frontiers in microbiology · 2019Article
- Identification and Analysis of a Novel Gene Cluster Involves in FeCurrent microbiology · 2018Article
- Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR.Scientific reports · 2018Article
- Article
- Can Sulfate Be the First Dominant Aqueous Sulfur Species Formed in the Oxidation of Pyrite byFrontiers in microbiology · 2018Article
- Molecular Systematics of the GenusFrontiers in microbiology · 2017Article
- Draft genome sequence of the type strain of the sulfur-oxidizing acidophile,Standards in genomic sciences · 2017Article
- Bioinformatic Analyses of Unique (Orphan) Core Genes of the GenusFrontiers in microbiology · 2016Article
- Regulation of Staphylococcal Superantigen-Like Gene, ssl8, Expression in Staphylococcus aureus strain, RN6390.Clinical medicine & research · 2015Article
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5 authors.
Funding
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Abstract
backgroundNormalization is a prerequisite for accurate real time PCR (qPCR) expression analysis and for the validation of microarray profiling data in microbial systems. The choice and use of reference genes that are stably expressed across samples, experimental conditions and designs is a key consideration for the accurate interpretation of gene expression data.
resultsHere, we evaluate a carefully selected set of reference genes derived from previous microarray-based transcriptional profiling experiments performed on Acidithiobacillus ferrooxidans and identify a set of genes with minimal variability under five different experimental conditions that are frequently used in Acidithiobacilli research. Suitability of these and other previously reported reference genes to monitor the expression of four selected target genes from A. ferrooxidans grown with different energy sources was investigated. Utilization of reference genes map, rpoC, alaS and era results in improved interpretation of gene expression profiles in A. ferrooxidans.
conclusionThis investigation provides a validated set of reference genes for studying A. ferrooxidans gene expression under typical biological conditions and an initial point of departure for exploring new experimental setups in this microorganism and eventually in other closely related Acidithiobacilli. The information could also be of value for future transcriptomic experiments in other bacterial systems.
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