ArticleBMC plant biology2009
Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 87 citations in OpenAlex.
- Advances in Genetic Transformation ofPlants (Basel, Switzerland) · 2026Review
- Triacontanol Boosts Soybean Nodulation viaPlants (Basel, Switzerland) · 2026Article
- Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The Agrobacterium-mediated genetic transformation: a gateway for efficient CRISPR/Cas9 gene editing in leguminous.Transgenic research · 2025Review
- Peas in Rouge: Tyrosine Supplementation Enhances RUBY Reporter Visibility inPlants (Basel, Switzerland) · 2025Article
- Screening of salt-tolerant soybean germplasm and study of salt-tolerance mechanism.Plant cell reports · 2025Article
- Loss of Lateral suppressor gene is associated with evolution of root nodule symbiosis in Leguminosae.Genome biology · 2024Article
- Genome-Wide Identification of the CDPK Gene Family and Their Involvement in Taproot Cracking in Radish.International journal of molecular sciences · 2023Article
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- The miR156b-GmSPL9d module modulates nodulation by targeting multiple core nodulation genes in soybean.The New phytologist · 2022Article
- Rhamnogalacturonan-I as a nematode chemoattractant fromFrontiers in plant science · 2022Article
- Genome-Wide Characterization of the Aquaporin Gene Family in Radish and Functional Analysis ofFrontiers in plant science · 2022Article
- Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV for genome editing.BMC plant biology · 2021Article
- Use of the rhizobial type III effector gene nopP to improve Agrobacterium rhizogenes-mediated transformation of Lotus japonicus.Plant methods · 2021Article
- An Optimized Transformation System and Functional Test ofInternational journal of molecular sciences · 2021Article
- GmYUC2a mediates auxin biosynthesis during root development and nodulation in soybean.Journal of experimental botany · 2019Article
- Genotype-independentPlant methods · 2018Article
- MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.Plant physiology · 2015Article
- Genome-Wide Small RNA Analysis of Soybean Reveals Auxin-Responsive microRNAs that are Differentially Expressed in Response to Salt Stress in Root Apex.Frontiers in plant science · 2015Article
- CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots.PloS one · 2015Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTransgenic approaches provide a powerful tool for gene function investigations in plants. However, some legumes are still recalcitrant to current transformation technologies, limiting the extent to which functional genomic studies can be performed on. Superroot of Lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. Recently, a technique to obtain transgenic L. corniculatus plants from Superroot-derived leaves through A. tumefaciens-mediated transformation was described. However, transformation efficiency was low and it took about six months from gene transfer to PCR identification.
resultsIn the present study, we developed an A. rhizogenes-mediated transformation of Superroot-derived L. corniculatus for gene function investigation, combining the efficient A. rhizogenes-mediated transformation and the rapid regeneration system of Superroot. The transformation system using A. rhizogenes K599 harbouring pGFPGUSPlus was improved by validating some parameters which may influence the transformation frequency. Using stem sections with one node as explants, a 2-day pre-culture of explants, infection with K599 at OD(600) = 0.6, and co-cultivation on medium (pH 5.4) at 22 degrees C for 2 days enhanced the transformation frequency significantly. As proof of concept, Superroot-derived L. corniculatus was transformed with a gene from wheat encoding an Na+/H+ antiporter (TaNHX2) using the described system. Transgenic Superroot plants were obtained and had increased salt tolerance, as expected from the expression of TaNHX2.
conclusionA rapid and efficient tool for gene function investigation in L. corniculatus was developed, combining the simplicity and high efficiency of the Superroot regeneration system and the availability of A. rhizogenes-mediated transformation. This system was improved by validating some parameters influencing the transformation frequency, which could reach 92% based on GUS detection. The combination of the highly efficient transformation and the regeneration system of Superroot provides a valuable tool for functional genomics studies in L. corniculatus.
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