ArticleProtein science : a publication of the Protein Society2009
Determination and application of empirically derived detergent phase boundaries to effectively crystallize membrane proteins.
Article in Protein science : a publication of the Protein Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- 20 years of crystal hits: progress and promise in ultrahigh-throughput crystallization screening.Acta crystallographica. Section D, Structural biology · 2023Article
- Crystallization of ApoA1 and ApoE4 nanolipoprotein particles and initial XFEL-based structural studies.Crystals · 2020Article
- Membrane association of monotopic phosphoglycosyl transferase underpins function.Nature chemical biology · 2018Article
- Crystal structure of rofecoxib bound to human cyclooxygenase-2.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Substrate-selective Inhibition of Cyclooxygeanse-2 by Fenamic Acid Derivatives Is Dependent on Peroxide Tone.The Journal of biological chemistry · 2016Article
- Manipulation of Subunit Stoichiometry in Heteromeric Membrane Proteins.Structure (London, England : 1993) · 2016Article
- The Crystal Structure of an Integral Membrane Fatty Acid α-Hydroxylase.The Journal of biological chemistry · 2015Article
- The detection and subsequent volume optimization of biological nanocrystals.Structural dynamics (Melville, N.Y.) · 2015Article
- Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Crystal structures of α-dioxygenase from Oryza sativa: insights into substrate binding and activation by hydrogen peroxide.Protein science : a publication of the Protein Society · 2013Article
- A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.Lab on a chip · 2013Article
- The crystal structure of α-Dioxygenase provides insight into diversity in the cyclooxygenase-peroxidase superfamily.Biochemistry · 2013Article
- The ESFRI Instruct Core Centre Frankfurt: automated high-throughput crystallization suited for membrane proteins and more.Journal of structural and functional genomics · 2012Article
- Crystal structure of a concentrative nucleoside transporter from Vibrio cholerae at 2.4 Å.Nature · 2012Article
- The transition from closed to open conformation of Treponema pallidum outer membrane-associated lipoprotein TP0453 involves membrane sensing and integration by two amphipathic helices.The Journal of biological chemistry · 2011Article
- Crystallization chaperone strategies for membrane proteins.Methods (San Diego, Calif.) · 2011Review
- Lessons from high-throughput protein crystallization screening: 10 years of practical experience.Expert opinion on drug discovery · 2011Article
- Pi sampling: a methodical and flexible approach to initial macromolecular crystallization screening.Acta crystallographica. Section D, Biological crystallography · 2011Article
- The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins.Journal of structural and functional genomics · 2010Article
- Effective critical micellar concentration of a zwitterionic detergent: a fluorimetric study on n-dodecyl phosphocholine.Journal of fluorescence · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Elucidating the structures of membrane proteins is essential to our understanding of disease states and a critical component in the rational design of drugs. Structural characterization of a membrane protein begins with its detergent solubilization from the lipid bilayer and its purification within a functionally stable protein-detergent complex (PDC). Crystallization of the PDC typically occurs by changing the solution environment to decrease solubility and promote interactions between exposed hydrophilic surface residues. As membrane proteins have been observed to form crystals close to the phase separation boundaries of the detergent used to form the PDC, knowledge of these boundaries under different chemical conditions provides a foundation to rationally design crystallization screens. We have carried out dye-based detergent phase partitioning studies using different combinations of 10 polyethylene glycols (PEG), 11 salts, and 11 detergents to generate a significant amount of chemically diverse phase boundary data. The resulting curves were used to guide the formulation of a 1536-cocktail crystallization screen for membrane proteins. We are making both the experimentally derived phase boundary data and the 1536 membrane screen available through the high-throughput crystallization facility located at the Hauptman-Woodward Institute. The phase boundary data have been packaged into an interactive Excel spreadsheet that allows investigators to formulate grid screens near a given phase boundary for a particular detergent. The 1536 membrane screen has been applied to 12 membrane proteins of unknown structures supplied by the structural genomics and structural biology communities, with crystallization leads for 10/12 samples and verification of one crystal using X-ray diffraction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.