ArticleJournal of experimental botany2009
Tobacco plastid transformation using the feedback-insensitive anthranilate synthase [alpha]-subunit of tobacco (ASA2) as a new selectable marker.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins.Plants (Basel, Switzerland) · 2022Review
- Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Review
- Vaccination via Chloroplast Genetics: Affordable Protein Drugs for the Prevention and Treatment of Inherited or Infectious Human Diseases.Annual review of genetics · 2016Review
- Expression of a Codon-Optimized dsdA Gene in Tobacco Plastids and Rice Nucleus Confers D-Serine Tolerance.Frontiers in plant science · 2016Article
- A mutant Synechococcus gene encoding glutamate 1-semialdehyde aminotransferase confers gabaculine resistance when expressed in tobacco plastids.Plant cell reports · 2015Article
- The Engineered Chloroplast Genome Just Got Smarter.Trends in plant science · 2015Review
- Plastid genomics in horticultural species: importance and applications for plant population genetics, evolution, and biotechnology.Frontiers in plant science · 2015Review
- Production of functional native human interleukin-2 in tobacco chloroplasts.Molecular biotechnology · 2014Article
- Plastid genetic engineering in Solanaceae.Protoplasma · 2012Review
- Chloramphenicol acetyltransferase as selectable marker for plastid transformation.Plant molecular biology · 2011Article
- Visual spectinomycin resistance (aadA(au)) gene for facile identification of transplastomic sectors in tobacco leaves.Plant molecular biology · 2011Article
- Over-expression of peptide deformylase in chloroplasts confers actinonin resistance, but is not a suitable selective marker system for plastid transformation.Transgenic research · 2011Article
- Plastid biotechnology: food, fuel, and medicine for the 21st century.Plant physiology · 2011Review
- Plant plastid engineering.Current genomics · 2010Article
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3 authors.
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Abstract
Genetic engineering of chloroplasts normally requires the stable introduction of bacterial derived antibiotic or herbicide-resistance genes as selective markers. Ecological and health concerns have been raised due to the presence of such genes within the environment or the food supply. One way to overcome this issue is the use of plant genes able to confer a metabolic or developmental advantage to the transformed cells manipulating the plant's biosynthetic pathways. We explored the feasibility of using, for plastid transformation, the selection system based on the feedback-insensitive anthranilate synthase (AS) alpha-subunit gene of tobacco (ASA2) as a new selective marker and the indole analogue 4-methylindole (4MI) or the tryptophan analogue 7-methyl-DL-tryptophan (7MT) as the selection agents. An expression cassette containing Prrn-ASA2 was effectively integrated into the region between accD and ycf4 of the tobacco plastome by the biolistic process. Plastid transgenic plants were obtained on medium supplemented with 300 microM 7MT or 4MI. Transplastomic plants showed normal phenotype and fertility and the resistance to the selection agents 7MT and 4MI was transmitted maternally. The plastid transformed lines also exhibited a higher level of AS enzyme activity that was less sensitive to Trp-feedback inhibition and, consequently, increased free Trp levels in leaves about 7-fold.
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