ArticleRNA (New York, N.Y.)2009
Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Overview on Strategies and Assays for Antibiotic Discovery.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Article
- Evaluation of Aminoglycoside and Carbapenem Resistance in a Collection of Drug-Resistant Pseudomonas aeruginosa Clinical Isolates.Microbial drug resistance (Larchmont, N.Y.) · 2018Article
- Antimicrobial Resistance inMicrobiology spectrum · 2018Review
- A tRNA-derived fragment competes with mRNA for ribosome binding and regulates translation during stress.RNA biology · 2017Article
- Techniques for Screening Translation Inhibitors.Antibiotics (Basel, Switzerland) · 2016Review
- Classic reaction kinetics can explain complex patterns of antibiotic action.Science translational medicine · 2015Article
- New trends in aminoglycosides use.MedChemComm · 2014Article
- Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology.Journal of biomolecular NMR · 2013Article
- Allosteric control of the ribosome by small-molecule antibiotics.Nature structural & molecular biology · 2012Article
- Proteins from an unevolved library of de novo designed sequences bind a range of small molecules.ACS synthetic biology · 2012Article
- Identification of protein-protein interaction inhibitors targeting vaccinia virus processivity factor for development of antiviral agents.Antimicrobial agents and chemotherapy · 2011Article
- Identification of distinct thiopeptide-antibiotic precursor lead compounds using translation machinery assays.Chemistry & biology · 2009Article
- Selection of small peptides, inhibitors of translation.Journal of molecular biology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Measuring the binding of antibiotics and other small-molecular-weight ligands to the 2.5 MDa ribosome often presents formidable challenges. Here, we describe a general method for studying binding of ligands to ribosomes that carry a site-specific fluorescent label covalently attached to one of the ribosomal proteins. As a proof of principle, an environment-sensitive fluorescent group was placed at several specific sites within the ribosomal protein S12. Small ribosomal subunits were reconstituted from native 16S rRNA, individually purified small subunit proteins, and fluorescently labeled S12. The fluorescence characteristics of the reconstituted subunits were affected by several antibiotics, including streptomycin and neomycin, which bind in the vicinity of protein S12. The equilibrium dissociation constants of the drugs obtained using a conventional fluorometer were in good agreement with those observed using previously published methods and with measurements based on the use of radiolabeled streptomycin. The newly developed method is rapid and sensitive, and can be used for determining thermodynamic and kinetic binding characteristics of antibiotics and other small ribosomal ligands. The method can readily be adapted for use in high-throughput screening assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.